SPECIFIC TARGETING OF MITOMYCIN-C TO TUMORS BY ANTIGASTRIC CANCER MONOCLONAL-ANTIBODY

被引：0

作者：

LI, S ^{[1
]}

ZHANG, XY ^{[1
]}

CHEN, XT ^{[1
]}

FAN, DM ^{[1
]}

ZHANG, SY ^{[1
]}

CHEN, LJ ^{[1
]}

SHU, YH ^{[1
]}

ZHANG, JL ^{[1
]}

机构：

[1] CHINESE ACAD SCI,SHANGHAI INST MAT MED,SHANGHAI 200031,PEOPLES R CHINA

来源：

CHINESE MEDICAL JOURNAL | 1991年 / 104卷 / 05期

关键词：

D O I：

暂无

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

In the present study, an anti-gastric cancer monoclonal antibody, MGb2, was chosen to prepare antibody-mitomycin C (MMC) conjugate with dextran T-40 as intermediary. Twenty molecules of MMC were introduced into each molecule of antibody while the antigen-binding capacity of the antibody was kept well. The conjugate showed selective cytotoxicity upon human gastric cancer cell line SGC-7901. Radioimmunoimaging and biodistribution studies indicated that after conjugation with MMC via dextran T-40 as intermediary, the tumor localization capacity of the antibody was well retained. When tested in nude mice, inoculated with human gastric carcinoma SGC-7901 in bilateral subrenal capsules, intraperitoneal injection of the conjugate daily for 6 days at a dose of 1 mg/kg gave a tumor inhibitory rate of 68.67%, which was far better than that of free MMC or irrelevant conjugate. No synergetic effect was found in regard to the mixture of MGb2 with MMC.

引用

页码：358 / 362

页数：5

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