MOLECULAR-CLONING AND SEQUENCE DETERMINATION OF 4 DIFFERENT CDNA SPECIES CODING FOR ALPHA-SUBUNITS OF G-PROTEINS FROM XENOPUS-LAEVIS OOCYTES

被引:49
作者
OLATE, J
MARTINEZ, S
PURCELL, P
JORQUERA, H
CODINA, J
BIRNBAUMER, L
ALLENDE, JE
机构
[1] UNIV NACL COLOMBIA, DEPT QUIM, SANTA FE DE BOGOTA, COLOMBIA
[2] BAYLOR UNIV, TEXAS MED CTR, DEPT CELL BIOL, HOUSTON, TX 77030 USA
关键词
cDNA cloning; G-protein; Nucleotide sequence; Xenopus laevis oocyte;
D O I
10.1016/0014-5793(90)80964-K
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A cDNA library preprared from Xenopus laevis oocytes in λgt10 was screened with a mixture of three oligonucleotide probes designed to detect sequences found in different mammalian genes coding for a-subunits of G-proteins. In addition to a clone coding for a Gαo-type subunit previously reported [(1989) FEBS Lett. 244, 188-192] four additional clones have been found coding for different Gα protein subunits. By comparison with mammalian α-subunits, these oocyte cDNAs correspond to two closely related Gas-la, to a Gαi-1 and to a Gαi-3 species. The derived amino acid sequences showed that both Gαs species contain 379 residues, corresponding to the short species without the serine residue and with a calculated Mr of 42720. The Gαi-1 gene encodes a 354 amino acid protein with an Mr, of 39000 and the Gαi-3 encodes an incomplete open reading frame of 345 residues, lacking the first 9 amino acid residues at the NH2, terminus. All these Gα-subunits showed high identity with their respective mammalian counterparts (75-80%), indicating a great degree of conservation through the evolution and the important cellular regulatory function that they play. © 1990.
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收藏
页码:27 / 31
页数:5
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