CD8 IS NEEDED FOR DEVELOPMENT OF CYTOTOXIC T-CELLS BUT NOT HELPER T-CELLS

被引：492
作者：
FUNGLEUNG, WP
SCHILHAM, MW
RAHEMTULLA, A
KUNDIG, TM
VOLLENWEIDER, M
POTTER, J
VANEWIJK, W
MAK, TW
机构：
[1] UNIV TORONTO, DEPT MED BIOPHYS, TORONTO M4X 1K9, ONTARIO, CANADA
[2] UNIV TORONTO, DEPT IMMUNOL, TORONTO M4X 1K9, ONTARIO, CANADA
[3] UNIV ZURICH, INST PATHOL, CH-8006 ZURICH, SWITZERLAND
[4] ERASMUS UNIV, DEPT IMMUNOL, 3000 DR ROTTERDAM, NETHERLANDS
来源：
CELL | 1991年 / 65卷 / 03期
基金：
英国医学研究理事会;
关键词：
D O I：
10.1016/0092-8674(91)90462-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A mutant mouse strain without CD8 (Lyt-2 and Lyt-3) expression on the cell surface has been generated by disrupting the Lyt-2 gene using embryonic stem cell technology. In these mice, CD8+ T lymphocytes are not present in peripheral lymphoid organs, but the CD4+ T lymphocyte population seems to be unaltered. Cytotoxic response of T lymphocytes from these mice against alloantigens and viral antigens is dramatically decreased. Proliferative response against alloantigens and in vivo help to B lymphocytes, however, are not affected. These data suggest that CD8 is necessary for the maturation and positive selection of class I MHC restricted cytotoxic T lymphocytes but is not required on any of the intermediate thymocyte populations (CD8+CD4-TcR- or CD4+CD8+TcR(low)) during the development of functional class II MHC restricted helper T cells.
引用
收藏
页码：443 / 449
页数：7
相关论文
共 51 条
[41]   SITE-DIRECTED MUTAGENESIS BY GENE TARGETING IN MOUSE EMBRYO-DERIVED STEM-CELLS [J].
THOMAS, KR ;
CAPECCHI, MR .
CELL, 1987, 51 (03) :503-512
[42]   GENES OF THE T-CELL ANTIGEN RECEPTOR IN NORMAL AND MALIGNANT T-CELLS [J].
TOYONAGA, B ;
MAK, TW .
ANNUAL REVIEW OF IMMUNOLOGY, 1987, 5 :585-620
[43]   INTERACTION OF THE UNIQUE N-TERMINAL REGION OF TYROSINE KINASE P56LCK WITH CYTOPLASMIC DOMAINS OF CD4 AND CD8 IS MEDIATED BY CYSTEINE MOTIFS [J].
TURNER, JM ;
BRODSKY, MH ;
IRVING, BA ;
LEVIN, SD ;
PERLMUTTER, RM ;
LITTMAN, DR .
CELL, 1990, 60 (05) :755-765
[44]   MONOCLONAL-ANTIBODIES TO STROMAL CELL-TYPES OF THE MOUSE THYMUS [J].
VANVLIET, E ;
MELIS, M ;
VANEWIJK, W .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1984, 14 (06) :524-529
[45]   THE CD4 AND CD8 T-CELL SURFACE-ANTIGENS ARE ASSOCIATED WITH THE INTERNAL MEMBRANE TYROSINE-PROTEIN KINASE P56LCK [J].
VEILLETTE, A ;
BOOKMAN, MA ;
HORAK, EM ;
BOLEN, JB .
CELL, 1988, 55 (02) :301-308
[46]   ENGAGEMENT OF CD4 AND CD8 EXPRESSED ON IMMATURE THYMOCYTES INDUCES ACTIVATION OF INTRACELLULAR TYROSINE PHOSPHORYLATION PATHWAYS [J].
VEILLETTE, A ;
ZUNIGAPFLUCKER, JC ;
BOLEN, JB ;
KRUISBEEK, AM .
JOURNAL OF EXPERIMENTAL MEDICINE, 1989, 170 (05) :1671-1680
[47]   LY ANTIGENS ASSOCIATED WITH T-CELL RECOGNITION AND EFFECTOR FUNCTION [J].
WALKER, ID ;
HOGARTH, PM ;
MURRAY, BJ ;
LOVERING, KE ;
CLASSON, BJ ;
CHAMBERS, GW ;
MCKENZIE, IFC .
IMMUNOLOGICAL REVIEWS, 1984, 82 :47-77
[48]   COMPARISON OF THYMIC AND PERIPHERAL T-CELL LY-2/3 ANTIGENS [J].
WALKER, ID ;
MURRAY, BJ ;
HOGARTH, PM ;
KELSO, A ;
MCKENZIE, IFC .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1984, 14 (10) :906-910
[49]   A HUMAN T-CELL-SPECIFIC CDNA CLONE ENCODES A PROTEIN HAVING EXTENSIVE HOMOLOGY TO IMMUNOGLOBULIN-CHAINS [J].
YANAGI, Y ;
YOSHIKAI, Y ;
LEGGETT, K ;
CLARK, SP ;
ALEKSANDER, I ;
MAK, TW .
NATURE, 1984, 308 (5955) :145-149
[50]   2 LYT-2 POLYPEPTIDES ARISE FROM A SINGLE GENE BY ALTERNATIVE SPLICING PATTERNS OF MESSENGER-RNA [J].
ZAMOYSKA, R ;
VOLLMER, AC ;
SIZER, KC ;
LIAW, CW ;
PARNES, JR .
CELL, 1985, 43 (01) :153-163
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