VARIABLE EXPRESSION OF O-ANTIGEN AND THE ROLE OF LIPOPOLYSACCHARIDE AS AN ADHESIN IN AEROMONAS-SOBRIA

被引：16
|
作者：
FRANCKI, KT [1 ]
CHANG, BJ [1 ]
机构：
[1] UNIV WESTERN AUSTRALIA,DEPT MICROBIOL,NEDLANDS,WA 6009,AUSTRALIA
来源：
FEMS MICROBIOLOGY LETTERS | 1994年 / 122卷 / 1-2期
关键词：
AEROMONAS SOBRIA; LIPOPOLYSACCHARIDE; ADHESION;
D O I：
10.1111/j.1574-6968.1994.tb07150.x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
On initial isolation of Aeromonas sobria 3767 from a diarrhoeal stool specimen, two colony types were obtained: opaque (3767O) and translucent (3767T). Strain 3767O consistently produced lipopolysaccharide (LPS) core and O-antigen side chain, detectable by SDS-PAGE and by Western blotting with an O-antigen-specific monoclonal antibody. Strain 3767T produced LPS core but the amount of O-antigen was dependent on factors including growth medium and bacterial growth phase. Strain 3767T exhibited significantly lower levels of adhesion to HEp-2 cells than 3767O and this correlated with the level of LPS expression, with the greatest reduction (61%) at stationary phase when no LPS was detectable. The results implicate LPS as an adhesin for A. sobria 3767.
引用
收藏
页码：97 / 101
页数：5
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