Comparison of DNA histograms by standard flow cytometry and image cytometry on sections in Barrett's adenocarcinoma

被引：16

作者：

Huang, Qin ^{[1
,2
,3
]}

Yu, Chenggong ^{[4
]}

Zhang, Xiaoqi ^{[4
]}

Goyal, Raj ^{[4
]}

机构：

[1] VA Boston Healthcare Syst, Dept Pathol, Providence, RI 02901 USA

[2] Providence VA Med Ctr, Providence, RI 02132 USA

[3] Brown Med Sch, Providence, RI 02901 USA

[4] VA Boston Hlth Care Syst & Harvard Med Sch, Dept Internal Med, West Roxbury, MA 02132 USA

来源：

BMC CLINICAL PATHOLOGY | 2008年 / 8卷

关键词：

D O I：

10.1186/1472-6890-8-5

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background: The purpose of this study was to compare DNA histograms obtained by standard flow cytometry (FC) and high fidelity image cytometry on sections (ICS) in normal gastrointestinal mucosa and Barrett's adenocarcinoma (BAC). Conclusion: ICS detects DNA aneuploidy in all BAC samples while FC missed the diagnosis of aneuploidy in 29%. In addition, ICS provides more information on HI and G2 exceeding rates.

引用

页数：9

共 34 条

[1]

Alanen KA, 1998, ANAL QUANT CYTOL, V20, P178

[2] INTRATUMORAL VARIATIONS IN DNA DISTRIBUTION PATTERNS IN MAMMARY ADENOCARCINOMAS [J].

ASKENSTEN, UG ;

VONROSEN, AK ;

NILSSON, RS ;

AUER, GU .

CYTOMETRY, 1989, 10 (03) :326-333

[3] CYTOPHOTOMETRY [J].

AUER, G ;

ASKENSTEN, U ;

AHRENS, O .

HUMAN PATHOLOGY, 1989, 20 (06) :518-527

[4]

BACUS JW, 1994, MODERN PATHOL, V7, P652

[5] Interlaboratory reproducibility of semiautomated cell cycle analysis of flow cytometric DNA-histograms obtained from fresh material of 1,295 breast cancer cases [J].

Bergers, E ;

Montironi, R ;

vanDiest, PJ ;

Prete, E ;

Baak, JPA .

HUMAN PATHOLOGY, 1996, 27 (06) :553-560

[6] Tumour heterogeneity of DNA cell cycle variables in breast cancer measured by flow cytometry [J].

Bergers, E ;

vanDiest, PJ ;

Baak, JPA .

JOURNAL OF CLINICAL PATHOLOGY, 1996, 49 (11) :931-937

[7]

DANQUE POV, 1993, MODERN PATHOL, V6, P270

[8]

DRESSLER LG, 1989, SEMIN DIAGN PATHOL, V6, P55

[9] DNA abnormalities as marker of risk for progression of Barrett's esophagus to adenocarcinoma: Image cytometric DNA analysis in formalin-fixed tissues [J].

Fang, M ;

Lew, E ;

Klein, M ;

Sebo, T ;

Su, YY ;

Goyal, R .

AMERICAN JOURNAL OF GASTROENTEROLOGY, 2004, 99 (10) :1887-1894

[10]

FLEJOU JF, 1990, ANN PATHOL, V10, P161

← 1 2 3 4 →