AUTOANTIBODIES TO POLY(ADP-RIBOSE)POLYMERASE IN AUTOIMMUNE-DISEASES

被引：23

作者：

NEGRI, C ^{[1
]}

SCOVASSI, AI ^{[1
]}

CERINO, A ^{[1
]}

NEGRONI, M ^{[1
]}

BORZI, RM ^{[1
]}

MELICONI, R ^{[1
]}

FACCHINI, A ^{[1
]}

MONTECUCCO, CM ^{[1
]}

RICOTTI, GCBA ^{[1
]}

机构：

[1] CNR,IST GENET BIOCHIM & EVOLUZIONISTICA,VIA ABBIATEGRASSO 207,I-27100 PAVIA,ITALY

来源：

AUTOIMMUNITY | 1990年 / 6卷 / 03期

关键词：

Anti nuclear antibodies; Connective tissue diseases; DNA repair and transcription; ELISA; Poly(ADP-ribose)polymerase; Western Blot;

D O I：

10.3109/08916939009041040

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Antibodies to various nuclear proteins are frequently found in sera of patients affected by connective tissue diseases and other autoimmune diseases. We investigated the specificity of circulating autoantibodies to poly(ADP-ribose)polymerase (pADPRP) in different autoimmune and connective tissue diseases: Systemic Lupus Erythematosus (SLE), Rheumatoid Arthritis (RA), Progressive Systemic Sclerosis (PSS), Sjogren's Syndrome (SS), Undifferen-tiated Connective Tissue Disease (UCTD), Cryptogenic Fibrosing Alveolitis (CFA) and Sarcoidosis. pADPRP was purified from calf thymus. Antibody specificity was detected by ELISA, western blot and enzyme activity precipitation. Positive values (mean O.D. values + 3 S.D. of 36 normal controls) were obtained in 7/15 SLE patients, 1/18 RA patients, 1/30 PSS, 3/14 SS, 0/5 UCTD, 5/21 CFA and 4/25 Sarcoidosis. The positive sera also recognized the pADPRP protein when tested by western blot. Furthermore the enzyme activity was inhibited after immunoprecipitation by some highly positive sera. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：203 / 209

页数：7

共 22 条

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