DETERMINATION OF LIGNIN IN HERBACEOUS PLANTS BY AN IMPROVED ACETYL BROMIDE PROCEDURE

被引：337

作者：

IIYAMA, K ^{[1
]}

WALLIS, AFA ^{[1
]}

机构：

[1] CSIRO,DIV FORESTRY & FOREST PROD,PRIVATE BAG 10,CLAYTON,VIC 3168,AUSTRALIA

来源：

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE | 1990年 / 51卷 / 02期

关键词：

acetyl bromide; acid detergent lignin; cinnamic acids; grasses; hardwoods; Herbaceous plants; legumes; lignin; lignin determination; milled sample lignins; nitrobenzene oxidation; protein; softwoods; ultraviolet spectra;

D O I：

10.1002/jsfa.2740510202

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A modified acetyl bromide procedure is proposed for the spectrophotometric determination of lignin in herbaceous plants. The digestion with 25 % acetyl bromide (AcBr) in acetic acid at 70°C is improved by including 4 % perchloric acid in the digesting solutions. This allows for more rapid, complete digestion and the use of coarser samples. On the basis of infrared spectra and nitrobenzene oxidation data for milled sample lignins, the value of 20.0 g−1 litre cm−1 for the specific absorption coeficient of AcBr‐treated lignins of herbaceous plant samples gave lignin values which were consistent with nitrobenzene oxidation data, but were higher than those obtained by the acid detergent lignin method and the earlier AcBr method proposed for grasses. The lower lignin values obtained in the latter methods are considered to be due to partial lignin solubilisation in the sulphuric acid digestion. Copyright © 1990 John Wiley & Sons, Ltd

引用

页码：145 / 161

页数：17

共 17 条

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Hatfield, RD
Grabber, J
Ralph, J
Brei, K
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1999, 47 (02) : 628 - 632
[2] The acetyl bromide lignin method accurately quantitates lignin in forage
Fukushima, R. S.
Kerley, M. S.
Ramos, M. H.
Kallenbach, R. L.
ANIMAL FEED SCIENCE AND TECHNOLOGY, 2021, 276
[3] DETERMINATION OF LIGNIN IN THE PRESENCE OF ESTER-BOUND SUBSTITUTED CINNAMIC-ACIDS BY A MODIFIED ACETYL BROMIDE PROCEDURE
MORRISON, IM
STEWART, D
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1995, 69 (02) : 151 - 157
[4] Comparison of acetyl bromide lignin with acid detergent lignin and Klason lignin and correlation with in vitro forage degradability
Fukushima, Romualdo S.
Kerley, Monty S.
Ramos, Marcelo H.
Porter, James H.
Kallenbach, Robert L.
ANIMAL FEED SCIENCE AND TECHNOLOGY, 2015, 201 : 25 - 37
[5] Feasibility of using lignin isolated from forages by solubilization in acetyl bromide as a standard for lignin analyses
Fukushima, RS
Dehority, BA
JOURNAL OF ANIMAL SCIENCE, 2000, 78 (12) : 3135 - 3143
[6] Reactions of lignin model beta-aryl ethers with acetyl bromide
Lu, FC
Ralph, J
HOLZFORSCHUNG, 1996, 50 (04) : 360 - 364
[7] Use of Lignin Extracted from Different Plant Sources as Standards in the Spectrophotometric Acetyl Bromide Lignin Method
Fukushima, Romualdo S.
Kerley, Monty S.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2011, 59 (08) : 3505 - 3509
[8] Rapid, microscale, acetyl bromide-based method for high-throughput determination of lignin content in Arabidopsis thaliana
Chang, Xue Feng
Chandra, Richard
Berleth, Thomas
Beatson, Rodger P.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2008, 56 (16) : 6825 - 6834
[9] Genetic modification of lignin concentration affects fitness of perennial herbaceous plants
Casler, MD
Buxton, DR
Vogel, KP
THEORETICAL AND APPLIED GENETICS, 2002, 104 (01) : 127 - 131
[10] Extraction and isolation of lignin for utilization as a standard to determine lignin concentration using the acetyl bromide spectrophotometric method
Fukushima, RS
Hatfield, RD
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2001, 49 (07) : 3133 - 3139

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