THE GTP-BINDING PROTEIN RAN/TC4 IS REQUIRED FOR PROTEIN IMPORT INTO THE NUCLEUS

被引：688

作者：

MOORE, MS

BLOBEL, G

机构：

[1] Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021

来源：

NATURE | 1993年 / 365卷 / 6447期

关键词：

D O I：

10.1038/365661a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Two cytosolic fractions (A and B) from Xenopus oocytes are sufficient to support protein import into the nuclei of digitonin-permeabilized cells1. Fraction A recognizes the nuclear localization sequence (NLS) and binds the import substrate to the nuclear envelope, whereas fraction B mediates the subsequent passage of the bound substrate into the nucleus. Here we report that two interacting components are required for full fraction-B activity, purify one of these components to homogeneity, and show that it is the highly abundant GTP-binding protein Ran (Ras-related nuclear protein)/TC4.

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页码：661 / 663

页数：3

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