FLOW CYTOMETRIC ANALYSIS OF DNA PLOIDY AND S-PHASE FRACTION IN BREAST-CANCER USING CELLS OBTAINED BY EXVIVO FINE-NEEDLE ASPIRATION - AN OPTIMAL METHOD FOR SAMPLE COLLECTION

被引：0
|
作者：
ELIASEN, CA
OPITZ, LM
VAMVAKAS, EC
ESPIRITU, EC
MARSH, ER
ROSES, DF
HARRIS, MN
FEINER, HD
机构：
[1] NYU MED CTR,TISCH HOSP,DEPT PATHOL,560 1ST AVE,NEW YORK,NY 10016
[2] NYU MED CTR,DEPT SURG,NEW YORK,NY 10016
来源：
MODERN PATHOLOGY | 1991年 / 4卷 / 02期
关键词：
BREAST CANCER; FLOW CYTOMETRY; FINE-NEEDLE ASPIRATION; DNA PLOIDY; S-PHASE FRACTION; PROLIFERATION INDEX;
D O I：
暂无
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
A total of 203 primary invasive breast cancers were sampled by ex vivo fine-needle aspiration (FNA), directly yielding adequate single cell suspensions for flow cytometric DNA analysis in 194 (96%). Labor-intensive and time-consuming steps of mechanical and enzymatic cellular disaggregation required by the use of fresh, frozen, or paraffin-embedded tissue were avoided, thereby minimizing preparation time. Conservation of tumor tissue allowed for the sampling of very small breast cancers. DNA ploidy and S-phase fraction data were comparable to flow cytometric data reported in other breast cancer studied using various sampling methods. Ex vivo FNA is the easiest and fastest method for sampling breast cancers for flow cytometric DNA analysis.
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页码：196 / 200
页数：5
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