CLONING OF HUMAN-ALPHA-1(X) COLLAGEN DNA AND LOCALIZATION OF THE COL10A1 GENE TO THE Q21-Q22 REGION OF HUMAN CHROMOSOME-6

With consensus primers based upon the nucleotide sequence of the chicken alpha-1(X) collagen gene, we have used PCR with human genomic DNA as template to isolate a 289 bp fragment coding for part of the carboxyl non-triple helical domain of the human alpha-1(X) gene. We have demonstrated the presence of the sequence of the PCR clone within the human genome by partial sequence analysis of a 1 kb HindIII genomic DNA fragment that hybridized with the PCR clone. Furthermore, using the PCR clone as a probe for in situ hybridization of human metaphase chromosome spreads, and for Southern analysis of a panel of human-hamster somatic cell hybrid DNAs, we have assigned the locus for the alpha-1(X) gene to the q21-q22 region of human chromosome 6.