TRANSCRIPTION ACTIVATES RECA-PROMOTED HOMOLOGOUS PAIRING OF NUCLEOSOMAL DNA

被引：46

作者：

KOTANI, H ^{[1
]}

KMIEC, EB ^{[1
]}

机构：

[1] THOMAS JEFFERSON UNIV,JEFFERSON CANC INST,DEPT PHARMACOL,PHILADELPHIA,PA 19107

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1994年 / 14卷 / 03期

关键词：

D O I：

10.1128/MCB.14.3.1949

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RecA protein catalyzes the homologous pairing of a single-stranded circular DNA and a linear duplex DNA molecule. When the duplex is packaged into chromatin, formation of homologously paired complexes is blocked. We have established a system for studying the RecA-promoted reaction by using a duplex fragment containing a single-phased nucleosome. Under these conditions there is no reaction leading to formation of joint molecule complexes. However, transcription on the chromatin template activates the formation of complexes. Reaction is dependent on RNA synthesis and DNA sequence homology and proceeds regardless of the direction of transcription.

引用

页码：1949 / 1955

页数：7

共 30 条

[1] DEVELOPMENT OF THE PRIMARY ANTIBODY REPERTOIRE [J].

ALT, FW ;

BLACKWELL, TK ;

YANCOPOULOS, GD .

SCIENCE, 1987, 238 (4830) :1079-1087

[2] INVITRO REPLICATION THROUGH NUCLEOSOMES WITHOUT HISTONE DISPLACEMENT [J].

BONNEANDREA, C ;

WONG, ML ;

ALBERTS, BM .

NATURE, 1990, 343 (6260) :719-726

[3] THE ROLE OF STABLE COMPLEXES THAT REPRESS AND ACTIVATE EUKARYOTIC GENES [J].

BROWN, DD .

CELL, 1984, 37 (02) :359-365

[4] A NUCLEOSOME CORE IS TRANSFERRED OUT OF THE PATH OF A TRANSCRIBING POLYMERASE [J].

CLARK, DJ ;

FELSENFELD, G .

CELL, 1992, 71 (01) :11-22

[5] SUPERHELICAL STRESS AND NUCLEOSOME-MEDIATED REPRESSION OF 5S RNA GENE-TRANSCRIPTION INVITRO [J].

CLARK, DJ ;

WOLFFE, AP .

EMBO JOURNAL, 1991, 10 (11) :3419-3428

[6]

DEMURCIA G, 1980, NUCLEIC ACIDS RES, V8, P767

[7] DNA BENDING AND ITS RELATION TO NUCLEOSOME POSITIONING [J].

DREW, HR ;

TRAVERS, AA .

JOURNAL OF MOLECULAR BIOLOGY, 1985, 186 (04) :773-790

[8] CHROMATIN AS AN ESSENTIAL PART OF THE TRANSCRIPTIONAL MECHANISM [J].

FELSENFELD, G .

NATURE, 1992, 355 (6357) :219-224

[9]

GRIFFIN JA, 1992, MECHANISM EUKARYOTIC, P75

[10] NUCLEOSOMES - REGULATORS OF TRANSCRIPTION [J].

GRUNSTEIN, M .

TRENDS IN GENETICS, 1990, 6 (12) :395-400

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