EFFECTS OF FREEZING RATE, THAWING RATE, GEOMETRY OF SEMEN SAMPLE, AND DILUTION METHODS ON THE CRYOPRESERVATION OF RAM SPERMATOZOA IN THE ABSENCE OF GLYCEROL

被引：0

作者：

ABDELHAKEAM, AA ^{[1
]}

GRAHAM, EF ^{[1
]}

DEYO, RC ^{[1
]}

机构：

[1] UNIV MINNESOTA, DEPT ANIM SCI, ST PAUL, MN 55108 USA

来源：

CRYOLETTERS | 1992年 / 13卷 / 06期

关键词：

FREEZING RATE; THAWING RATE; GEOMETRY; DILUTION METHOD; RAM SPERMATOZOA;

D O I：

暂无

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The effects of freezing and thawing rates, sample geometry and dilution methods on the preservation of ram spermatozoa in the absence of glycerol were studied. Percentage of motile spermatozoa was recorded for unfrozen (5oC at 4 and 24 hr) and frozen-thawed (22oC at 0 and 4 hr) samples. The highest post-thaw motility was obtained with 0.25cc straws frozen at 73.8oC/min and thawed at 49oC/min in a 37oC water bath. Cold diluted semen yielded higher motility (P<0.05) for both unfrozen and frozen spermatozoa.

引用

页码：395 / 404

页数：10

共 33 条

[1]

ABDELHAKEAM A A, 1988, Journal of Animal Science, V66, P145

[2]

ABDELHAKEAM A A, 1988, Journal of Animal Science, V66, P146

[3]

Almquist J. O., 1973, A.I. Digest, V21, P10

[4] OPTIMUM THAWING TEMPERATURE AND TIME FOR BOVINE SEMEN PROCESSED BY 3 METHODS AND PACKAGED IN 1 ML AMPULES [J].

CHANDLER, JE ;

NEBEL, RL ;

ADKINSON, RW .

THERIOGENOLOGY, 1983, 19 (02) :201-212

[5]

COLAS G, 1975, J REPROD FERTIL, V42, P277, DOI 10.1530/jrf.0.0420277

[6] EFFECTS OF COMPOSITION OF DILUENT, METHOD OF ADDITION OF GLYCEROL, FREEZING RATE, AND STORAGE TEMPERATURE ON REVIVAL OF RAM SPERMATOZOA AFTER DEEP-FREEZING [J].

ENTWISTLE, KW ;

MARTIN, ICA .

AUSTRALIAN JOURNAL OF BIOLOGICAL SCIENCES, 1972, 25 (02) :379-+

[7] THE EFFECT OF GLYCEROL CONCENTRATION AND COOLING VELOCITY ON CRYOSURVIVAL OF RAM SPERMATOZOA FROZEN IN STRAWS [J].

FISER, PS ;

FAIRFULL, RW .

CRYOBIOLOGY, 1984, 21 (05) :542-551

[8] COMBINED EFFECTS OF GLYCEROL CONCENTRATION, COOLING VELOCITY, AND OSMOLALITY OF SKIM MILK DILUENTS ON CRYOPRESERVATION OF RAM SPERMATOZOA [J].

FISER, PS ;

FAIRFULL, RW .

THERIOGENOLOGY, 1986, 25 (03) :473-484

[9]

FOOTE RH, 1984, 10TH P NAAB TECHN C, P62

[10]

GOODNIGHT JH, 1979, SAS USERS GUIDE, P121

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