GROWTH AND DISSEMINATION OF HUMAN-MALIGNANT LYMPHOBLASTS IN IMMUNOSUPPRESSED NU NU MICE

被引：0

作者：

CAVALLO, F ^{[1
]}

RICCARDI, C ^{[1
]}

FORNI, M ^{[1
]}

PERICLE, F ^{[1
]}

BOSCO, MC ^{[1
]}

GIOVARELLI, M ^{[1
]}

SOLETI, A ^{[1
]}

FORNI, G ^{[1
]}

机构：

[1] UNIV TURIN,INST MICROBIOL,I-10124 TURIN,ITALY

来源：

NATURAL IMMUNITY AND CELL GROWTH REGULATION | 1991年 / 10卷 / 05期

关键词：

NU NU MICE; IMMUNOSUPPRESSION; HUMAN LEUKEMIA; NATURAL KILLER ACTIVITY; MENINGEAL DISSEMINATION;

D O I：

暂无

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Athymic nu/nu mice are commonly employed for the heterotransplantation of solid human tumors. Leukemias, however, have consistently proved difficult to transplant and, to enhance their take, recipient nu/nu mice have been variously immunosuppressed. In this study, the natural reactivity against human malignant T lymphoblast (PF382) of splenectomized nu/nu mice (S-nu/nu), nu/nu mice splenectomized and treated with polyinosinic-polycytidylic acid (SIC-nu/nu), and nu/nu mice splenectomized, irradiated and repeatedly injected with antiasialo GM1 antiserum (SIA-nu/nu) has been correlated with the in vivo growth of subcutaneous and intravenous PF382 cell challenges. SIC-nu/nu mice display a marked natural killer (NK) activity, quickly clear I-125-Urd-labelled PF382 cells injected intravenously and do not allow the growth of subcutaneous nor intravenous PF382 cell challenges. S-nu/nu mice display a slightly lower NK activity and slower clearance of I-125-Urd-labelled PF382 cells. Moreover, an intravenous PF382 cell challenge kills 56% of S-nu/nu mice. SIA-nu/nu mice have no NK activity, slowly clear I-125-Urd-labelled PF382 cells and always allow the growth of PF382 cells injected either subcutaneously or intravenously with a consistent pattern. Following the intravenous challenge, PF382 cells first metastasize to liver and kidney, then focal or diffuse infiltrations of the bone marrow and meninges become evident. SIA-nu/nu mice thus offer an interesting experimental model for study of the pathogenesis of leukemic infiltration of the meninges, and the exploration of possible therapeutic approaches.

引用

页码：256 / 264

页数：9