HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF RIBONUCLEOTIDES BY POSTCOLUMN DERIVATIZATION INVOLVING OXIDATION FOLLOWED BY FLUORESCENCE REACTION, AND ITS APPLICATION TO HUMAN ERYTHROCYTE SAMPLE

被引：4
作者：
UMEGAE, Y
NOHTA, H
OHKURA, Y
机构：
[1] Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka 812
来源：
ANALYTICAL SCIENCES | 1990年 / 6卷 / 04期
关键词：
1,2-bis(4-methoxyphenyl)ethylenediamine; erythrocyte; high performance liquid chromatography; periodate oxidation; postcolumn fluorescence derivatization; Ribonucleotide;
D O I：
10.2116/analsci.6.519
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A selective method for the determination of ribonucleotides is described which employs high performance liquid chromatography with on-line postcolumn fluorescence derivatization. Ribonucleotides are separated on a reversed-phased column (TSK gel ODS-8()tm) and automatically converted into fluorescent derivatives by reaction with l,2-bis(4-methoxyphenyl)ethylenediamine in an acidic medium after periodate oxidation. The detection limits (SjN= 3) for ribonucleotides are 14 - 67 pmol in a 100 pi injection volume. The quantification of ribonucleotides in human erythrocytes is also described. © 1990, The Japan Society for Analytical Chemistry. All rights reserved.
引用
收藏
页码：519 / 522
页数：4
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