IMPROVED SEPARATION OF MODIFIED NUCLEOSIDES FROM TRANSFER-RNA HYDROLYSATES - PATTERNS OF TRANSFER-RNA METHYLATION IN RAT-TISSUES

被引：4

作者：

CLARK, I

TREBILCOCKGUZMAN, MA

机构：

[1] Department of Surgery, College of Medicine and Dentistry of New Jersey, Rutgers Medical School, Piscataway, NJ 08854

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1979年 / 1卷 / 05期

基金：

美国国家科学基金会;

关键词：

high-performance liquid chromatography; rat tissue; tRNA methyltransferases;

D O I：

10.1016/0165-022X(79)90003-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive and reproducible method for the isolation of minor nucleosides derived from tRNA is described. The nucleosides obtained from enzymatic digestion of tRNA are separated into several groups using a QAE Sephadex column and increasing concentration of boric acid in a step-wise manner. The nucleosides in each group are separated by isocratic elution from a preparative Partisil 10-SCX column and high-performance liquid chromatography at ambient temperature. With this method we have determined the patterns of tRNA methylation in vitro with extracts from rat bone, liver, kidney and adrenal glands. Although different tissues appear to contain the same tRNA methyltransferases, the patterns of methylated nucleosides are different. © 1979.

引用

页码：287 / 298

页数：12

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