GLUTARALDEHYDE CYANURIC CHLORIDE AND TETRAAZOTIZED O-DIANISISDINE AS COUPLING REAGENTS IN PASSIVE HEMAGGLUTINATION TEST

Using tetraazotized o-dianisidine, cyanuric chloride or glutaraldehyde, antigens were coupled to erythrocytes. The sensitized cells could be used in passive hemagglunation tests. With the three named reagents the agglutination reaction was specific and clear cut pictures of positive or negative hemmagglutination were obtained. The maximum hemagglutination titers were at least as high as those obtained with erythrocytes sensitized with bis-diazotized benzidine or tannic acid. The cells coated with the acid of glutaraldehyde could be lyophilized and used again without any appreciable loss of maximum agglutination titer. © 1969.