QUANTIFICATION OF PROTEIN IN DILUTE AND COMPLEX SAMPLES - MODIFICATION OF THE BICINCHONINIC ACID ASSAY

被引：21

作者：

SCHOEL, B

WELZEL, M

KAUFMANN, SHE

机构：

[1] Department of Immunology, University of Ulm, D-89070 Ulm

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1995年 / 30卷 / 2-3期

关键词：

PROTEIN ASSAY; BICINCHONINIC ACID; INTERFERENCE;

D O I：

10.1016/0165-022X(95)00009-G

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The colorimetric assay using bicinchoninic acid (BCA) as test reagent is useful for quantitative protein determinations due to its high sensitivity, ease, and tolerance to various contaminations present in biological samples or added during purification. For removal of interfering substances, protein precipitations have been described. Yet, obstructions became apparent with diluted and complex samples. Therefore we tested different solvents for removal of such interfering contaminants from the protein precipitate, and 1 M HCl was identified as the useful washing agent. The protocol described allows simple and accurate microdetermination in microtiter plates of proteins from complex samples.

引用

页码：199 / 206

页数：8

共 15 条

[1] ASSAY OF PROTEINS IN PRESENCE OF INTERFERING MATERIALS [J].