CLASSIFICATION AND MEASUREMENT OF FUNGAL PELLETS BY AUTOMATED IMAGE-ANALYSIS

被引：101

作者：

COX, PW ^{[1
]}

THOMAS, CR ^{[1
]}

机构：

[1] UNIV BIRMINGHAM,SCH CHEM ENGN,EDGBASTON,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1992年 / 39卷 / 09期

关键词：

PELLET CHARACTERIZATION; PELLET MEASUREMENT; IMAGE ANALYSIS; ASPERGILLUS-NIGER;

D O I：

10.1002/bit.260390909

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An automated image analysis method for classifying and measuring pellets of filamentous fungi growing in submerged fermentations has been developed. The method discriminates between pelleted mycelial growth and loose aggregates of dispersed hyphae. Pellets are classified into smooth and hairy types. In both cases, the core of the pellet is identified and its shape and size characterized. For hairy pellets the annular region is also characterized. The method was tested on pellets of Aspergillus niger ATCC 11414 grown in a defined medium in shake flasks. This rapid method makes practical extensive studies on the morphology of pellets in submerged fermentations and the influence of fermentation conditions on that morphology.

引用

页码：945 / 952

页数：8

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