FUSION OF NEGATIVELY CHARGED PHOSPHOLIPID-VESICLES BY ALPHA-LATROTOXIN

被引：5
|
作者：
LISHKO, VK [1 ]
TERLETSKAYA, YT [1 ]
TRIKASH, IO [1 ]
机构：
[1] AV PALLADIN BIOCHEM INST,DEPT NEUROCHEM,9 LEONTOVITCH ST,KIEV 252030,UKRAINE,USSR
来源：
FEBS LETTERS | 1990年 / 266卷 / 1-2期
关键词：
Liposome; Membrane fusion; α-Latrotoxin;
D O I：
10.1016/0014-5793(90)81516-Q
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
α-Latrotoxin-induced fusion of liposomes has been described using large unilamellar vesicles composed of phosphatidylcholine/phosphatidylethanolamine/cardiolipin at a molar ratio of 2:3:5. Vesicle fusion was monitored by terbium/dipicolinic acid assay as well as by fluorescence energy transfer measurement. The enhancement of the fusogenic effect of LTX by low concentrations (0.1-3 mM) of CaCl2 has been demonstrated. The efficiency of other divalent cations on the LTX fusogenic activity was shown to decrease in the sequence Ca > Cd > Sr > Mg > Ba. LTX-induced fusion was accompanied by the increase of vesicle size measured by laser correlation spectroscopy. It is concluded that fusogenic action of LTX may be involved in its effect on synaptic apparatus. © 1990.
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页码：99 / 101
页数：3
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