PROTEIN DIFFUSION IN POROUS GEL-FILTRATION CHROMATOGRAPHY MEDIA STUDIED BY PULSED FIELD GRADIENT NMR-SPECTROSCOPY

被引：47

作者：

GIBBS, SJ ^{[1
]}

LIGHTFOOT, EN ^{[1
]}

ROOT, TW ^{[1
]}

机构：

[1] UNIV WISCONSIN,DEPT CHEM ENGN,MADISON,WI 53706

来源：

JOURNAL OF PHYSICAL CHEMISTRY | 1992年 / 96卷 / 18期

关键词：

D O I：

10.1021/j100197a060

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Ovalbumin intradiffusion (or tracer diffusion) coefficients within commercially available polymeric gel filtration particles have been determined by pulsed field gradient NMR (PFGNMR) as a function of ovalbumin concentration. The results are well correlated with behavior in free solution by a tortuosity factor of 2.2 +/- 0.4 for the entire concentration range studied. A method for restricting diffusing species to individual particles, to observe intraparticle behavior rather than interparticle transport, is described. PFGNMR is shown to be a powerful experimental technique for selectively observing diffusion of protein or other tracer species within opaque, porous particles.

引用

页码：7458 / 7462

页数：5

共 35 条

[1] ANALYSIS OF AFFINITY SEPARATIONS .2. THE CHARACTERIZATION OF AFFINITY COLUMNS BY PULSE TECHNIQUES [J].