PROTEIN DIFFUSION IN POROUS GEL-FILTRATION CHROMATOGRAPHY MEDIA STUDIED BY PULSED FIELD GRADIENT NMR-SPECTROSCOPY

被引：47
作者：
GIBBS, SJ [1 ]
LIGHTFOOT, EN [1 ]
ROOT, TW [1 ]
机构：
[1] UNIV WISCONSIN,DEPT CHEM ENGN,MADISON,WI 53706
来源：
JOURNAL OF PHYSICAL CHEMISTRY | 1992年 / 96卷 / 18期
关键词：
D O I：
10.1021/j100197a060
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Ovalbumin intradiffusion (or tracer diffusion) coefficients within commercially available polymeric gel filtration particles have been determined by pulsed field gradient NMR (PFGNMR) as a function of ovalbumin concentration. The results are well correlated with behavior in free solution by a tortuosity factor of 2.2 +/- 0.4 for the entire concentration range studied. A method for restricting diffusing species to individual particles, to observe intraparticle behavior rather than interparticle transport, is described. PFGNMR is shown to be a powerful experimental technique for selectively observing diffusion of protein or other tracer species within opaque, porous particles.
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收藏
页码：7458 / 7462
页数：5
相关论文
共 35 条
  • [1] ANALYSIS OF AFFINITY SEPARATIONS .2. THE CHARACTERIZATION OF AFFINITY COLUMNS BY PULSE TECHNIQUES
    ARNOLD, FH
    BLANCH, HW
    WILKE, CR
    [J]. CHEMICAL ENGINEERING JOURNAL AND THE BIOCHEMICAL ENGINEERING JOURNAL, 1985, 30 (02): : B25 - B36
  • [2] MODELING AND ANALYSIS OF BIOSPECIFIC ADSORPTION IN A FINITE BATH
    ARVE, BH
    LIAPIS, AI
    [J]. AICHE JOURNAL, 1987, 33 (02) : 179 - 193
  • [3] PREDICTABILITY OF CHROMATOGRAPHIC PROTEIN SEPARATIONS - STUDY OF SIZE-EXCLUSION MEDIA WITH NARROW PARTICLE-SIZE DISTRIBUTIONS
    ATHALYE, AM
    GIBBS, SJ
    LIGHTFOOT, EN
    [J]. JOURNAL OF CHROMATOGRAPHY, 1992, 589 (1-2): : 71 - 85
  • [4] DIFFRACTION-LIKE EFFECTS IN NMR DIFFUSION STUDIES OF FLUIDS IN POROUS SOLIDS
    CALLAGHAN, PT
    COY, A
    MACGOWAN, D
    PACKER, KJ
    ZELAYA, FO
    [J]. NATURE, 1991, 351 (6326) : 467 - 469
  • [5] HIGH-RESOLUTION Q-SPACE IMAGING IN POROUS STRUCTURES
    CALLAGHAN, PT
    MACGOWAN, D
    PACKER, KJ
    ZELAYA, FO
    [J]. JOURNAL OF MAGNETIC RESONANCE, 1990, 90 (01): : 177 - 182
  • [6] DIFFUSION AND PARTITIONING OF MACROMOLECULES WITHIN FINELY POROUS-GLASS
    COLTON, CK
    LAI, CJ
    SATTERFIELD, CN
    [J]. AICHE JOURNAL, 1975, 21 (02) : 289 - 298
  • [7] DETERMINATION OF DIFFUSION-COEFFICIENTS OF PROTEINS IN BEADED AGAROSE BY GEL-FILTRATION
    DAVIES, PA
    [J]. JOURNAL OF CHROMATOGRAPHY, 1989, 483 : 221 - 237
  • [8] HINDERED TRANSPORT OF LARGE MOLECULES IN LIQUID-FILLED PORES
    DEEN, WM
    [J]. AICHE JOURNAL, 1987, 33 (09) : 1409 - 1425
  • [9] EPSTEIN N, 1988, CHEM ENG SCI, V44, P777
  • [10] PRACTICAL MODELS FOR ISOTHERMAL DIFFUSION AND FLOW OF GASES IN POROUS SOLIDS
    FENG, C
    STEWART, WE
    [J]. INDUSTRIAL & ENGINEERING CHEMISTRY FUNDAMENTALS, 1973, 12 (12): : 143 - 147
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