ASP-56, A NEW ACTIN SEQUESTERING PROTEIN FROM PIG PLATELETS WITH HOMOLOGY TO CAP, AN ADENYLATE CYCLASE-ASSOCIATED PROTEIN FROM YEAST

被引：70
|
作者：
GIESELMANN, R
MANN, K
机构：
[1] TECH UNIV MUNICH,DEPT BIOPHYS,W-8046 GARCHING,GERMANY
[2] MAX PLANCK INST BIOCHEM,W-8033 MARTINSRIED,GERMANY
来源：
FEBS LETTERS | 1992年 / 298卷 / 2-3期
关键词：
ACTIN-SEQUESTERING PROTEIN; ACTIN; ADENYLATE CYCLASE-ASSOCIATED PROTEIN;
D O I：
10.1016/0014-5793(92)80043-G
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A new 56 kDa actin-binding protein (ASP-56) was isolated from pig platelet lysate. In falling ball viscosimetry it caused a reduction in viscosity that could be attributed to a decrease in the concentration of polymeric actin. Fluorescence measurements with NBD-labelled actin showed reduction of polymeric actin, too. These results could be explained by sequestering of actin in a non-polymerizable 1:1 ASP-56/actin complex. Sequencing of about 20 tryptic peptides of ASP-56 and comparison with known sequences revealed about 60% homology to the adenylate cyclase-associated protein (CAP) from yeast.
引用
收藏
页码：149 / 153
页数：5
相关论文
共 50 条
  • [21] Cyclase-associated protein 1 (CAP1) promotes cofilin-induced actin dynamics in mammalian nonmuscle cells
    Bertling, E
    Hotulainen, P
    Mattila, PK
    Matilainen, T
    Salminen, M
    Lappalainen, P
    MOLECULAR BIOLOGY OF THE CELL, 2004, 15 (05) : 2324 - 2334
  • [22] Mammalian Adenylyl Cyclase-associated Protein 1 (CAP1) Regulates Cofilin Function, the Actin Cytoskeleton, and Cell Adhesion
    Zhang, Haitao
    Ghai, Pooja
    Wu, Huhehasi
    Wang, Changhui
    Field, Jeffrey
    Zhou, Guo-Lei
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2013, 288 (29) : 20966 - 20977
  • [23] Disease association of cyclase-associated protein (CAP): Lessons from gene-targeted mice and human genetic studies
    Rust, Marco B.
    Marcello, Elena
    EUROPEAN JOURNAL OF CELL BIOLOGY, 2022, 101 (02)
  • [24] Cyclase-associated Protein (CAP) Acts Directly on F-actin to Accelerate Cofilin-mediated Actin Severing across the Range of Physiological pH
    Normoyle, Kieran P. M.
    Brieher, William M.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (42) : 35722 - 35732
  • [25] Mammalian CAP1 (adenylyl Cyclase-Associated Protein 1) Regulates Cofilin function, the Actin Cytoskeleton and Cell Adhesion.
    Zhang, H.
    Ghai, P.
    Field, J.
    Zhou, G-L.
    MOLECULAR BIOLOGY OF THE CELL, 2012, 23
  • [26] Native cyclase-associated protein and actin from Xenopus laevis oocytes form a unique 4:4 complex with a tripartite structure
    Kodera, Noriyuki
    Abe, Hiroshi
    Nguyen, Phuong Doan N.
    Ono, Shoichiro
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2021, 296
  • [27] Human cyclase-associated protein (CAP1) regulates barbed end actin dynamics using its WH2 domain
    Alimov, N.
    Goode, B. L.
    MOLECULAR BIOLOGY OF THE CELL, 2023, 34 (02) : 459 - 460
  • [28] Mammalian homolog of the yeast cyclase associated protein, CAP/Srv2p, regulates actin filament assembly
    Freeman, NL
    Field, J
    CELL MOTILITY AND THE CYTOSKELETON, 2000, 45 (02): : 106 - 120
  • [29] Loss of the actin regulator cyclase-associated protein 1 (CAP1) modestly affects dendritic spine remodeling during synaptic plasticity
    Heinze, Anika
    Rust, Marco B.
    EUROPEAN JOURNAL OF CELL BIOLOGY, 2023, 102 (04)
  • [30] A high affinity interaction with ADP-actin monomers underlies the mechanism and in vivo function of Srv2/cyclase-associated protein (CAP)
    Quintero-Monzon, O
    Mattila, PK
    Kugler, J
    Moseley, JB
    Almo, SC
    Lappalainen, P
    Goode, BL
    MOLECULAR BIOLOGY OF THE CELL, 2004, 15 : 148A - 148A
12345