ASP-56, A NEW ACTIN SEQUESTERING PROTEIN FROM PIG PLATELETS WITH HOMOLOGY TO CAP, AN ADENYLATE CYCLASE-ASSOCIATED PROTEIN FROM YEAST

被引：70

作者：

GIESELMANN, R

MANN, K

机构：

[1] TECH UNIV MUNICH,DEPT BIOPHYS,W-8046 GARCHING,GERMANY

[2] MAX PLANCK INST BIOCHEM,W-8033 MARTINSRIED,GERMANY

来源：

FEBS LETTERS | 1992年 / 298卷 / 2-3期

关键词：

ACTIN-SEQUESTERING PROTEIN; ACTIN; ADENYLATE CYCLASE-ASSOCIATED PROTEIN;

D O I：

10.1016/0014-5793(92)80043-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new 56 kDa actin-binding protein (ASP-56) was isolated from pig platelet lysate. In falling ball viscosimetry it caused a reduction in viscosity that could be attributed to a decrease in the concentration of polymeric actin. Fluorescence measurements with NBD-labelled actin showed reduction of polymeric actin, too. These results could be explained by sequestering of actin in a non-polymerizable 1:1 ASP-56/actin complex. Sequencing of about 20 tryptic peptides of ASP-56 and comparison with known sequences revealed about 60% homology to the adenylate cyclase-associated protein (CAP) from yeast.

引用

页码：149 / 153

页数：5

共 19 条

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[2]

DETMERS P, 1981, J BIOL CHEM, V256, P99

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