CATECHOLAMINE LEVELS AND TURNOVER DURING AGING IN BRAIN-REGIONS OF MALE C57BL-6J MICE

被引：80

作者：

OSTERBURG, HH ^{[1
]}

DONAHUE, HG ^{[1
]}

SEVERSON, JA ^{[1
]}

FINCH, CE ^{[1
]}

机构：

[1] UNIV SO CALIF, DEPT BIOL SCI, LOS ANGELES, CA 90007 USA

来源：

BRAIN RESEARCH | 1981年 / 224卷 / 02期

关键词：

D O I：

10.1016/0006-8993(81)90864-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A radioenzymatic assay is described for measuring brain catecholamines (CA) and 3,4-dihydroxyphenylacetic acid (DOPAC) in the same tissue extract. The [3H]-methylated products are differentially extracted and then acetylated by acetic anhydride, followed by thin layer chromatography in non-basic solvents. Routine sensitivity is 3-5 pg per sample. This assay gave CA levels in brain regions which generally agreed with previous reports. CA levels/mg protein and turnover (after injection of .alpha.-methyl-p-tyrosine) were measured in male C57BL/6J mice at various ages between 4-30 mo., the average lifespan of male C57BL/6J mice. No region showed progressive age changes in levels or turnover, or changes before midlife, 8-12 mo. Brain regions with no evidence of age changes include globus pallidus, zona incerta, substantia nigra, cerebellum and olfactory bulbs. Small changes (10-25%) were detected in only some regions of mice .gtoreq. 24 mo.; few changes were statistically significant. CA levels and turnover decreased in some samplings of striatum and median eminence-arcuate nucleus. DA levels increased in anterior pituitary and possibly in medial preoptic region. DOPAC levels (measured only in rostral striatum) decreased by 20% at 28 mo. The results diverge from the larger, progressive decreases of DA reported in human striatum during aging.

引用

页码：337 / 352

页数：16

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