EPR AND REDOX PROPERTIES OF DESULFOVIBRIO-VULGARIS MIYAZAKI HYDROGENASE - COMPARISON WITH THE NI-FE ENZYME FROM DESULFOVIBRIO-GIGAS

被引：49
作者：
ASSO, M
GUIGLIARELLI, B
YAGI, T
BERTRAND, P
机构：
[1] UNIV AIX MARSEILLE 1,CTR ST JEROME,ELECTR MILIEUX CONDENSES LAB,CNRS,URA 784,F-13397 MARSEILLE 4,FRANCE
[2] SHIZUOKA UNIV,DEPT CHEM,SHIZUOKA,JAPAN
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1122卷 / 01期
关键词：
HYDROGENASE; EPR; NICKEL CENTER; IRON-SULFUR CLUSTER;
D O I：
10.1016/0167-4838(92)90126-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have carried out a detailed redox titration monitored by EPR on the hydrogenase from Desulfovibrio vulgaris Miyazaki. Typical 3Fe and nickel signals have been observed, which are very similar to those given by Desulfovibrio gigas hydrogenase in all the characteristic redox states of the enzyme. This confirms that D. vulgaris Miyazaki hydrogenase is a Ni-Fe enzyme closely related to that from D. gigas, as was recently proposed on the basis of sequence comparisons (Deckers, H.M., Wilson, F.R. and Voordouw, G. (1990) J. Gen. Microb. 136, 2021-2028).
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页码：50 / 56
页数：7
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