Fast Separation of Nicotinamide and Nicotinic Acid with Molecularly Imprinted Monolithic Column

Nicotinamide has been employed as template for the preparation of molecularly imprinted monolithic polymer (MIP) which was used as liquid chromatographic stationary phase. It showed that the nicotinamide-MIP monolithic column was capable of recognizing the difference between nicotinamide and nicotinic acid, while the non-imprinted polymer had no such ability. The content of organic solvent, flow rate and pH of the mobile phase were investigated in the experiments. The following high performance liquid chromatographic conditions were selected: column, 50 mm x 4. 6 mm i. d.; mobile phase, water; flow rate, 7. 0 mL/min; experiment temperature, room temperature. The resolution between nicotinamide and nicotinic acid was 1. 8. The results showed that the two compounds could be separated by the nicotinamide-MIP monolithic column. The column is very useful for the enrichment and detection of nicotinamide in body fluids.