NYLON-LINAMARASE ELECTRODE FOR RAPID-DETERMINATION OF LINAMARIN

被引：16

作者：

YEOH, HH

机构：

[1] Department of Botany, National University of Singapore, Kent Ridge, Singapore

来源：

BIOTECHNOLOGY TECHNIQUES | 1993年 / 7卷 / 10期

关键词：

D O I：

10.1007/BF00152627

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An enzyme electrode was constructed using cassava leaf linamarase covalently linked via polyethyleneimine to Hybond-N nylon. The nylon-enzyme electrode response was Nerstian for linamarin range of 0.1 to 20 mM. A steady state reading could be obtained within 4 to 6 mins. The nylon-enzyme discs could be reused. Compared to the previously reported enzyme electrode prepared by entrappment of linamarase in ENT-4000 prepolymer resins, the nylon-enzyme electrode gave faster response and could save analysis time by 60%.

引用

收藏

页码：761 / 764

页数：4

相关论文

共 9 条

[1] IMMOBILIZATION OF ENZYMES TO NYLON FILM [J].

ANDREWS, AT ;

MBAFOR, W .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1991, 19 (03) :S271-S271

[2] RAPID SCREENING ASSAY OF CYANIDE CONTENT OF CASSAVA [J].

BRADBURY, JH ;

EGAN, SV .

PHYTOCHEMICAL ANALYSIS, 1992, 3 (02) :91-94

[3] ANALYSIS OF CYANIDE IN CASSAVA USING ACID-HYDROLYSIS OF CYANOGENIC GLUCOSIDES [J].

BRADBURY, JH ;

EGAN, SV ;

LYNCH, MJ .

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1991, 55 (02) :277-290

[4] ENZYMATIC ASSAY FOR TOTAL CYANIDE CONTENT OF CASSAVA (MANIHOT-ESCULENTA CRANTZ) [J].

COOKE, RD .

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1978, 29 (04) :345-352

[5] STUDIES ON CASSAVA MANIHOT UTILISSIMA POHL - .I. CYANOGENESIS - BIOSYNTHESIS OF LINAMARIN AND LOTAUSTRALIN IN ETIOLATED SEEDLINGS [J].

NARTEY, F .

PHYTOCHEMISTRY, 1968, 7 (08) :1307-&

[6] ISOLATION PROPERTIES AND ENZYMIC BREAKDOWN OF LINAMARIN FROM CASSAVA [J].

WOOD, T .

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1966, 17 (02) :85-&

[7] QUANTITATIVE-ANALYSIS OF LINAMARIN IN CASSAVA USING A CASSAVA BETA-GLUCOSIDASE ELECTRODE [J].

YEOH, HH ;

TRUONG, VD .

FOOD CHEMISTRY, 1993, 47 (03) :295-298

[8] KINETIC-PROPERTIES OF BETA-GLUCOSIDASE FROM CASSAVA [J].

YEOH, HH .

PHYTOCHEMISTRY, 1989, 28 (03) :721-724

[9]

YEOH HH, 1992, BIOTECHNOL APPL BIOC, V15, P221