A c-type cytochrome, cytochrome c-550, was highly purified from a moderately thermophilic bacterium PS3. The absorption peaks were at 550, 521, and 416 nm in the reduced form, and at 408 nm in the oxidized form. The isoelectric point was at pH 5.6, and the molecular weight was about 10,000. The heat stability of the cytochrome was intermediate between those of horse cytochrome c and cytochrome c-552 from an extreme thermophile, Thermus thermophilus HB8. © 1979 Academic Press, Inc. All rights of reproduction in any form reserved.