LARVAL MEMBRANE-ANTIGENS PROTECT HEREFORD CATTLE AGAINST INFESTATION WITH BOOPHILUS-MICROPLUS

Summary Hereford cattle (Bos taurus) were immunized with antigens solubilized with Triton X‐100 from larval membranes of the cattle tick (Boophilus microplus). Based on tick egg production compared to control cattle, vaccinated cattle were protected (78%) against challenge with 2 × 20000 tick larvae. The soluble Triton X‐100 extract of tick larval membranes was further purified by immunoaffinity chromatography, using immunoglobulin ligands (IgG1 and IgG2) from three immune steers, previously vaccinated with membrane antigens from the midgut of partly engorged adult female ticks. Cattle vaccinated with these purified antigens were protected in two separate experiments (80 and 89% respectively), against challenge with 2 × 20000 larval ticks compared to control cattle. Whole larval membranes used as vaccines in cattle reduced the amount of eggs produced from ticks by 47% compared to control cattle, but this difference was not significant. Copyright © 1990, Wiley Blackwell. All rights reserved