STEREOSPECIFIC ANALYSIS OF FISH OIL TRIACYL-SN-GLYCEROLS

被引：44

作者：

ANDO, Y ^{[1
]}

NISHIMURA, K ^{[1
]}

AOYANAGI, N ^{[1
]}

TAKAGI, T ^{[1
]}

机构：

[1] HOKKAIDO UNIV,DEPT CHEM,FAC FISHERIES,MINATO CHO,HAKODATE,HOKKAIDO 041,JAPAN

来源：

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY | 1992年 / 69卷 / 05期

关键词：

CHIRAL STATIONARY PHASE; FISH OIL; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; MONOACYLGLYCEROLS; STEREOSPECIFIC ANALYSIS; TRIACYL-SN-GLYCEROLS;

D O I：

10.1007/BF02540941

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Stereospecific analysis of fish oil triacyl-sn-glycerols was carried out by novel high-performance liquid chromatography on a chiral stationary phase The Positional distributions of fatty acids were determined without accumulation of errors in a particular position and preferential hydrolysis for a particular fatty acid. High-resolution gas-liquid chromatography on an open tubular column detailed the distribution of unsaturated fatty acid isomers having olefinic bonds in different positions. The distribution of fatty acids was not independent of other fatty acids. The distribution of long-chain highly unsaturated fatty acids 22:6(n-3), 22:5(n-3) and 20:5(n-3) was governed by total amounts of 20:1 and 22:1 in triacyl-sn-glycerols. Long chain monounsaturated acids 20:1 and 22:1 were influenced by the position of the olefinic bond in 20:1 occurring in triacyl-sn-glycerols. Shorter-chain C14-C18 fatty acids seemed to be influenced by total fatty acid composition. These results introduce a concept of mutual interaction between fatty acids to the investigation of positional distribution of fatty acids.

引用

页码：417 / 424

页数：8

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