DEVELOPMENT OF PLASMID CLONING VECTORS FOR THERMUS-THERMOPHILUS HB8 - EXPRESSION OF A HETEROLOGOUS, PLASMID-BORNE KANAMYCIN NUCLEOTIDYLTRANSFERASE GENE

被引：42

作者：

MATHER, MW ^{[1
]}

FEE, JA ^{[1
]}

机构：

[1] UNIV CALIF LOS ALAMOS SCI LAB,STABLE ISOTOPE RESOURCE ISOTOPE & STRUCT CHEM GRP,LOS ALAMOS,NM 87545

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1992年 / 58卷 / 01期

关键词：

D O I：

10.1128/AEM.58.1.421-425.1992

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

While several Thermus genes have been cloned and T. thermophilus has been shown to be transformable, molecular genetic studies of these thermophiles have been hampered by the absence of selectable cloning vectors. We have constructed a selectable plasmid by random insertion of a heterologous gene encoding a thermostable kanamycin nucleotidyltransferase activity into a cryptic, multicopy plasmid from T. thermophilus HB8. This plasmid should serve as a suitable starting point for the development of a gene expression system for T. thermophilus.

引用

页码：421 / 425

页数：5

共 27 条

[1]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[2] NUCLEOTIDE-SEQUENCE OF THE PHOSPHOGLYCERATE KINASE GENE FROM THE EXTREME THERMOPHILE THERMUS-THERMOPHILUS - COMPARISON OF THE DEDUCED AMINO-ACID SEQUENCE WITH THAT OF THE MESOPHILIC YEAST PHOSPHOGLYCERATE KINASE [J].