IDENTIFICATION AND CHARACTERIZATION OF NOVEL HUMAN ENDOGENOUS RETROVIRAL SEQUENCES PREFERENTIALLY EXPRESSED IN UNDIFFERENTIATED EMBRYONAL CARCINOMA-CELLS

被引：0
作者：
LAMANTIA, G
MAGLIONE, D
PENGUE, G
DICRISTOFANO, A
SIMEONE, A
LANFRANCONE, L
LANIA, L
机构：
[1] NAPLES UNIV,DEPT GENET GEN & MOLEC BIOL,VIA MEZZOCANNONE 8,I-80124 NAPLES,ITALY
[2] CNR,INT INST GENET & BIOPHYS,NAPLES,ITALY
[3] UNIV PERUGIA,INST MED CLIN 1,I-06100 PERUGIA,ITALY
来源：
NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 07期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel endogenous retroviral sequence (ERV-9) has been isolated from a human embryonal carcinoma cDNA library by hybridization to a probe containing a recently described human repetitive element. DNA sequence analysis of the 4kb cDNA insert (pHE.1) revealed the presence of ORFs potentially coding for putative retrovirus-related gag, pol and env proteins. Northern blot and RNase protection experiments showed that RNA homologous to the pHE.1 insert is detected only in embryonal carcinoma cells as a 8 kb mRNA, and its expression is negatively regulated during retinoic acid induced differentiation of the human teratocarcinoma cell line NT2/D1. Using a pol specific probe we have isolated a genomic locus containing the ERV-9 sequences. Characterization by restriction enzyme analysis and DNA sequencing allowed us to define LTR-like sequences, that are composed by a complex array of subrepetitive elements. In addition we show that ERV-9 LTR sequences are capable to drive expression of linked CAT gene in a cell specific manner as LTR promoter activity has been detected only in NT2/D1 cells.
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页码：1513 / 1520
页数：8
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