SPECIFIC ALU BINDING-PROTEIN FROM HUMAN SPERM CHROMATIN PREVENTS DNA METHYLATION

被引：44
作者：
CHESNOKOV, IN
SCHMID, CW
机构：
[1] UNIV CALIF DAVIS, MOLEC & CELLULAR BIOL PROGRAM, DAVIS, CA 95616 USA
[2] UNIV CALIF DAVIS, DEPT CHEM, DAVIS, CA 95616 USA
来源：
JOURNAL OF BIOLOGICAL CHEMISTRY | 1995年 / 270卷 / 31期
关键词：
D O I：
10.1074/jbc.270.31.18539
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A protein from human sperm nuclei that specifically binds to Alu DNA repeats has been purified. The specific DNA binding site of this protein within the Alu sequence has been mapped by methylation interference and electrophoretic mobility shift assays. This sperm Alu binding protein selectively protects Alu elements from methylation in vitro and may be responsible for the unmethylated state of Alu sequences in the male germ line resulting in a parent-specific differential inheritance of Alu methylation.
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页码：18539 / 18542
页数：4
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