DIVERSITY OF ETHANOL-TOLERANT LACTOBACILLI ISOLATED FROM DOURO FORTIFIED WINE - CLUSTERING AND IDENTIFICATION BY NUMERICAL-ANALYSIS OF ELECTROPHORETIC PROTEIN PROFILES

被引：27

作者：

COUTO, JA ^{[1
]}

HOGG, TA ^{[1
]}

机构：

[1] UNIV CATOLICA PORTUGUESA, ESCOLA SUPER BIOTECNOL, P-4200 Porto, PORTUGAL

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1994年 / 76卷 / 05期

关键词：

D O I：

10.1111/j.1365-2672.1994.tb01106.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Eighty-two ethanol-tolerant (greater than or equal to 15% v/v) isolates of Lactobacillus were obtained from Douro fortified wines and associated winery equipment. The diversity and specific identity of these isolates was studied with computer-assisted comparison of the whole-cell, SDS-solubilized protein profiles (as generated by SDS-PAGE); with this method four species were identified. The majority of isolates (89%) were identified as Lact. hilgardii, and three differentiable electrophoretic groups of this species were evident. The identification was confirmed by DNA-DNA dot-blot hybridization studies. Also identified were one strain each of Lact. fructivorans, Lact. collinoides and Lact. mall.

引用

页码：487 / 491

页数：5

共 21 条

[1] ECOLOGY AND CLASSIFICATION OF STRAINS OF LACTOBACILLUS-COLLINOIDES NOV-SPEC - BACTERIUM COMMONLY FOUND IN FERMENTING APPLE JUICE
CARR, JG
DAVIES, PA
[J]. JOURNAL OF APPLIED BACTERIOLOGY, 1972, 35 (03): : 463 - &
[2] RELATIONSHIP BETWEEN LACTOBACILLUS-MALI FROM CIDER AND LACTOBACILLUS-YAMANASHIENSIS FROM WINE
CARR, JG
DAVIES, PA
DELLAGLIO, F
VESCOVO, M
WILLIAMS, RAD
[J]. JOURNAL OF APPLIED BACTERIOLOGY, 1977, 42 (02): : 219 - 228
[3] LACTOBACILLI CAUSING SPOILAGE OF ACETIC ACID PRESERVES
DAKIN, JC
RADWELL, JY
[J]. JOURNAL OF APPLIED BACTERIOLOGY, 1971, 34 (03): : 541 - &
[4] RELATEDNESS OF HETEROFERMENTATIVE LACTOBACILLUS SPECIES REVEALED BY NUMERICAL-ANALYSIS OF TOTAL SOLUBLE CELL PROTEIN-PATTERNS
DICKS, LMT
VANVUUREN, HJJ
[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1987, 37 (04): : 437 - 440
[5] FORNACHON J, 1949, BACTERIAL SPOILAGE F
[6] Goswell R. W., 1986, Developments in Food Microbiology, V2, P1
[7] Hammes W. P., 1992, The prokaryotes: a handbook on the biology of bacteria: ecophysiology, isolation, identification, applications, vol. II., P1535
[8] JACKMAN P, 1985, CHEM METHODS BACTERI, P188
[9] Kandler O., 1986, BERGEYS MANUAL SYSTE, P1208
[10] IDENTIFICATION AND GROUPING OF BACTERIA BY NUMERICAL-ANALYSIS OF THEIR ELECTROPHORETIC PROTEIN PATTERNS
KERSTERS, K
DELEY, J
[J]. JOURNAL OF GENERAL MICROBIOLOGY, 1975, 87 (APR): : 333 - 342

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