DIVERSITY OF ETHANOL-TOLERANT LACTOBACILLI ISOLATED FROM DOURO FORTIFIED WINE - CLUSTERING AND IDENTIFICATION BY NUMERICAL-ANALYSIS OF ELECTROPHORETIC PROTEIN PROFILES

被引：27

作者：

COUTO, JA ^{[1
]}

HOGG, TA ^{[1
]}

机构：

[1] UNIV CATOLICA PORTUGUESA, ESCOLA SUPER BIOTECNOL, P-4200 Porto, PORTUGAL

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1994年 / 76卷 / 05期

关键词：

D O I：

10.1111/j.1365-2672.1994.tb01106.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Eighty-two ethanol-tolerant (greater than or equal to 15% v/v) isolates of Lactobacillus were obtained from Douro fortified wines and associated winery equipment. The diversity and specific identity of these isolates was studied with computer-assisted comparison of the whole-cell, SDS-solubilized protein profiles (as generated by SDS-PAGE); with this method four species were identified. The majority of isolates (89%) were identified as Lact. hilgardii, and three differentiable electrophoretic groups of this species were evident. The identification was confirmed by DNA-DNA dot-blot hybridization studies. Also identified were one strain each of Lact. fructivorans, Lact. collinoides and Lact. mall.

引用

页码：487 / 491

页数：5

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