PURIFICATION OF RECOMBINANT HEPATITIS DELTA-ANTIGEN EXPRESSED IN ESCHERICHIA-COLI-CELLS

被引:3
作者
CALOGERO, R
BARBIERI, U
BORLA, M
OSBORNE, S
POISSON, F
BONELLI, F
机构
[1] SORIN BIOMED SPA,DIV R&D DIAGNOST,I-13040 SALUGGIA,ITALY
[2] CHU BRETONNEAU,CNRS,URA 1334,F-37044 TOURS,FRANCE
关键词
HEPATITIS DELTA-ANTIGEN; FUSION PROTEIN; PURIFICATION;
D O I
10.1016/0014-5793(93)80538-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Recombinant DNA technology enables the massive production of recombinant hepatitis delta antigen (recHDAg) retaining immunological properties and transport functions. However, purification procedures of the recombinant delta antigen have, to date, not been described in the literature, We present a purification procedure allowing one to obtain highly purified recHDAg from bacterial cells expressing the hepatitis delta antigen.
引用
收藏
页码:322 / 324
页数:3
相关论文
共 50 条
[21]   α-Conotoxin as Potential to α7-nAChR Recombinant Expressed in Escherichia coli [J].
Liu, Yanli ;
Yin, Yifeng ;
Song, Yunyang ;
Wang, Kang ;
Wu, Fanghui ;
Jiang, Hui .
MARINE DRUGS, 2020, 18 (08)
[22]   FRAGMENTATION AND CHARACTERISTICS OF RECOMBINANT ENDOCHITINASE EXPRESSED IN ESCHERICHIA COLI [J].
Chen, Wei-Ming ;
Chen, Gen-Hung ;
Jiang, Shann-Tzong .
JOURNAL OF MARINE SCIENCE AND TECHNOLOGY-TAIWAN, 2012, 20 (04) :369-375
[23]   Purification and Refolding of Cyclodextrin Glycosyltransferase Expressed Escherichia coli [J].
Dae-Hyukkweon Kweon ;
Sung-Gunkim Kim ;
Jin-Hoseo Seo .
Journal of inclusion phenomena and macrocyclic chemistry, 2004, 50 :37-41
[24]   High yield refolding and purification process for recombinant human interleukin-6 expressed in Escherichia coli [J].
Ejima, D ;
Watanabe, M ;
Sato, Y ;
Date, M ;
Yamada, N ;
Takahara, Y .
BIOTECHNOLOGY AND BIOENGINEERING, 1999, 62 (03) :301-310
[25]   Recombinant production and purification of novel antisense antimicrobial peptide in Escherichia coli [J].
Haught, C ;
Davis, GD ;
Subramanian, R ;
Jackson, KW ;
Harrison, RG .
BIOTECHNOLOGY AND BIOENGINEERING, 1998, 57 (01) :55-61
[26]   Facile purification of Escherichia coli expressed tag-free recombinant human tumor necrosis factor alpha from supernatant [J].
Zhang, Chun ;
Liu, Yongdong ;
Zhao, Dawei ;
Li, Xiunan ;
Yu, Rong ;
Su, Zhiguo .
PROTEIN EXPRESSION AND PURIFICATION, 2014, 95 :195-203
[27]   Multiple optimizations of recombinant plasmid for improving expression of Hepatitis B core antigen in Escherichia coli [J].
Zhang, Yi ;
Guo, Minli ;
Zhang, Xinyue ;
Zhang, Nan ;
Zhu, Ping ;
Wang, Hao .
PROTEIN EXPRESSION AND PURIFICATION, 2022, 198
[28]   Production and Purification of Recombinant Proteins from Escherichia coli [J].
Tripathi, Nagesh K. .
CHEMBIOENG REVIEWS, 2016, 3 (03) :116-133
[29]   In vitro and in vivo activities of recombinant anthrax protective antigen co-expressed with thioredoxin in Escherichia coli [J].
Ma, Yao ;
Yu, Yun-Zhou ;
Zhu, Yu-Feng ;
Xu, Qing ;
Sun, Zhi-Wei .
HUMAN VACCINES & IMMUNOTHERAPEUTICS, 2013, 9 (11) :2371-2377
[30]   Purification and structural analysis of the hepatitis B virus PreS1 expressed from Escherichia coli [J].
Maeng, CY ;
Oh, MS ;
Park, IH ;
Hong, HJ .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 282 (03) :787-792