PURIFICATION OF RECOMBINANT HEPATITIS DELTA-ANTIGEN EXPRESSED IN ESCHERICHIA-COLI-CELLS

被引：3
|
作者：
CALOGERO, R
BARBIERI, U
BORLA, M
OSBORNE, S
POISSON, F
BONELLI, F
机构：
[1] SORIN BIOMED SPA,DIV R&D DIAGNOST,I-13040 SALUGGIA,ITALY
[2] CHU BRETONNEAU,CNRS,URA 1334,F-37044 TOURS,FRANCE
来源：
FEBS LETTERS | 1993年 / 318卷 / 03期
关键词：
HEPATITIS DELTA-ANTIGEN; FUSION PROTEIN; PURIFICATION;
D O I：
10.1016/0014-5793(93)80538-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Recombinant DNA technology enables the massive production of recombinant hepatitis delta antigen (recHDAg) retaining immunological properties and transport functions. However, purification procedures of the recombinant delta antigen have, to date, not been described in the literature, We present a purification procedure allowing one to obtain highly purified recHDAg from bacterial cells expressing the hepatitis delta antigen.
引用
收藏
页码：322 / 324
页数：3
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