PRELIMINARY CRYSTALLOGRAPHIC STUDIES OF THE AMINO TERMINAL HALF OF HUMAN LACTOFERRIN IN ITS IRON-SATURATED AND IRON-FREE FORMS

被引：7
作者：
DAY, CL
NORRIS, GE
ANDERSON, BF
TWEEDIE, JW
BAKER, EN
机构：
[1] Department of Chemistry, Biochemistry Massey University Palmerston North
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 228卷 / 03期
关键词：
HUMAN LACTOFERRIN; AMINO TERMINAL; CRYSTALLIZATION; IRON-SATURATED; IRON-FREE;
D O I：
10.1016/0022-2836(92)90880-S
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The amino terminal half of human lactoferrin (LfN) produced from transfected baby hamster kidney cells has been crystallized in its iron-saturated and iron-free forms. The crystals of glycosylated LfN and deglycosylated LfN are monoclinic, space group C2, with cell dimensions a = 133·0 A ̊, b = 58·3 A ̊, c = 58·3 A ̊, α = 90·0 °, β = 114·7 °, γ = 90·0 °, and one molecule per asymmetric unit. Crystals of apo LfN have also been prepared using deglycosylated protein. These crystals are tetragonal, space group P41212 (or P43212), with cell dimensions of a = b = 58·4 A ̊ and c = 217·2 A ̊ and one molecule per asymmetric unit. Both the iron-saturated and the iron-free crystals are suitable for high resolution X-ray analysis. © 1992.
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页码：973 / 974
页数：2
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