CORRELATION OF COFACTOR BINDING AND THE QUATERNARY STRUCTURE OF PYRUVATE DECARBOXYLASE AS REVEALED BY P-31 NMR-SPECTROSCOPY

被引：10

作者：

HUBNER, G ^{[1
]}

KONIG, S ^{[1
]}

SCHNACKERZ, KD ^{[1
]}

机构：

[1] UNIV WURZBURG,THEODOR BOVERI INST BIOWISSENSCH,W-8700 WURZBURG,GERMANY

来源：

FEBS LETTERS | 1992年 / 314卷 / 01期

关键词：

PYRUVATE DECARBOXYLASE; THIAMINE PYROPHOSPHATE; P-31 NMR SPECTROSCOPY;

D O I：

10.1016/0014-5793(92)81471-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The pH dependence of the quaternary structure of pyruvate decarboxylase (EC 4.1.1.1) has recently been discovered [(1990) FEBS Lett. 266, 17-20; (1992) Biochemistry (in press)]. In the present study we have investigated the change in quaternary structure by observing the binding of the cofactor, thiamine pyrophosphate, using P-31 NMR spectroscopy. The dissociation of the native tetramers into dimers when increasing the pH coincides with a weaker binding of the cofactor and loss of enzyme activity. The results provide further evidence that thiamine pyrophosphate is bound primarily via the beta-phosphate moiety. In addition, a phosphoserine has been discovered in two of the four subunits.

引用

页码：101 / 103

页数：3