SOLID-PHASE RADIOIMMUNOASSAY FOR THE DETECTION OF ANTIBODIES TO COLLAGEN

被引：30
作者：
CLAGUE, RB
BROWN, RA
WEISS, JB
LENNOXHOLT, PJ
机构：
[1] Department of Rheumatology, University of Manchester, Manchester, Stopford Building
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1979年 / 27卷 / 01期
关键词：
D O I：
10.1016/0022-1759(79)90236-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A solid-phase radioimmunoassay has been developed using polystyrene tubes for the detection of class-specific antibodies to collagen. The optimal conditions for the adsorption of collagen onto the tubes, followed by incubation with the antisera and finally the radiolabelled antibody to the class-specific antibody are described. Studies carried out on antisera raised in rats and rabbits using this assay confirm its sensitivity and applicability. © 1979.
引用
收藏
页码：31 / 41
页数：11
相关论文
共 23 条
[1]   SENSITIVE RADIOIMMUNOASSAY FOR COLLAGEN [J].
ADELMANN, BC ;
GENTNER, GJ ;
HOPPER, K .
JOURNAL OF IMMUNOLOGICAL METHODS, 1973, 3 (04) :319-336
[2]  
BEIL W, 1973, IMMUNOLOGY, V24, P13
[3]   PREPARATION OF TYPE-III PROCOLLAGEN AND COLLAGEN FROM RAT SKIN [J].
BYERS, PH ;
MCKENNEY, KH ;
LICHTENSTEIN, JR ;
MARTIN, GR .
BIOCHEMISTRY, 1974, 13 (25) :5243-5248
[4]   SOLID-PHASE RADIOIMMUNOASSAY IN ANTIBODY-COATED TUBES [J].
CATT, K ;
TREGEAR, GW .
SCIENCE, 1967, 158 (3808) :1570-&
[5]   COLLAGEN POLYMORPHISM - CHARACTERIZATION OF MOLECULES WITH CHAIN COMPOSITION [ALPHA-1(III)]3 IN HUMAN TISSUES [J].
CHUNG, E ;
MILLER, EJ .
SCIENCE, 1974, 183 (4130) :1200-1201
[6]   DIFFERENT ANTIGENIC DETERMINANTS IN POLYPEPTIDE CHAINS OF HUMAN COLLAGEN [J].
FURTHMAYR, H ;
BEIL, W ;
TIMPL, R .
FEBS LETTERS, 1971, 12 (06) :341-+
[7]  
FURTHMAYR H, 1975, INT REV CONNECTIVE T, V7, P61
[8]  
GUNSON DE, 1976, IMMUNOLOGY, V31, P563
[9]  
HAHN E, 1974, J IMMUNOL, V113, P421
[10]   ROUTINE ASSAY FOR DETECTION OF IGG AND IGM ANTIGLOBULINS IN SERONEGATIVE AND SEROPOSITIVE RHEUMATOID-ARTHRITIS [J].
HAY, FC ;
NINEHAM, LJ ;
ROITT, IM .
BRITISH MEDICAL JOURNAL, 1975, 3 (5977) :203-204
123