AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR IGG ANTIBODIES TO HUMAN HERPES VIRUS-6

被引：26
作者：
DAHL, H [1 ]
LINDE, A [1 ]
SUNDQVIST, VA [1 ]
WAHREN, B [1 ]
机构：
[1] STOCKHOLM COLL HLTH & CARING SCI,DEPT MED LAB TECHNOL,STOCKHOLM,SWEDEN
来源：
JOURNAL OF VIROLOGICAL METHODS | 1990年 / 29卷 / 03期
关键词：
ELISA; HHV6; antibodies; IFA;
D O I：
10.1016/0166-0934(90)90058-N
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An indirect enzyme-linked immunosorbent assay (ELISA) with human herpes virus 6 (HHV6) membrane antigen was compared with indirect immunofluorescence assay (IFA) for measurement of HHV6 IgG antibodies. Five hundred serum samples from 403 Swedish patients with suspected symptomatic Epstein-Barr virus (EBV) infections were examined. The specificity of the ELISA compared with IFA was 98.7% and the sensitivity was 98.4%. In 90% of the patients, IgG antibodies to HHV6 were detected with both assays. The highest HHV6 IgG titers were found mainly in patients with EBV or CMV infections, but HHV6 mononucleosis was not diagnosed. The same HHV6 antigen was assessed for IgM ELISA but was found to be of limited value due to high IgM reactivity with the control antigen. The HHV6 IgM ELISA requires further investigation. The IgG ELISA described is a reliable alternative to IFA for measurement of HHV6 IgG antibodies and for large scale epidemiological studies. © 1990.
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页码：313 / 324
页数：12
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