ENZYMATIC DETERMINATION OF TOTAL CHOLESTEROL IN SERUM BY FLOW-INJECTION ANALYSIS

被引：6

作者：

FERNANDEZROMERO, JM ^{[1
]}

DECASTRO, MDL ^{[1
]}

VALCARCEL, M ^{[1
]}

机构：

[1] UNIV CORDOBA, FAC SCI, DEPT ANALYT CHEM, CORDOBA, SPAIN

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1987年 / 5卷 / 04期

关键词：

D O I：

10.1016/0731-7085(87)80039-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Several methods are proposed for the enzymatic determination of total cholesterol in serum by flow injection analysis (FIA). The use of the merging-zones mode, with or without halting of the reacting plug in the photometric or fluorimetric detector, has allowed the development of four precise methods for the determination of this analyte; calibration curves were linear for ranges of 0.02-0.2 and 0.005-0.05 gl-1. These methods have been applied to the determination of cholesterol in serum with excellent results.

引用

页码：333 / 340

页数：8

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