ATOMIC-FORCE MICROSCOPY OF SINGLE-STRANDED AND DOUBLE-STRANDED DEOXYRIBONUCLEIC-ACID

被引：47
作者：
THUNDAT, T
ALLISON, DP
WARMACK, RJ
DOKTYCZ, MJ
JACOBSON, KB
BROWN, GM
机构：
[1] OAK RIDGE NATL LAB,DIV BIOL,OAK RIDGE,TN 37831
[2] OAK RIDGE NATL LAB,DIV CHEM,OAK RIDGE,TN 37831
来源：
JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A-VACUUM SURFACES AND FILMS | 1993年 / 11卷 / 04期
关键词：
D O I：
10.1116/1.578312
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
The atomic force microscope (AFM) was used to image double-stranded DNA and single-stranded DNA adsorbed onto mica modified by Ba (II). Images of double-stranded DNA show an open circular shape without drastic contortions, and contour lengths that measure within 7% of the calculated length. Images of single-stranded DNA show two distinct forms; compact open circles with ''nodes'' or lumps almost uniformly distributed are the most frequently observed while less abundant are highly elongated circles with a few nodes. Heating the single-stranded DNA sample and altering the solvent conditions prior to adsorption was found to increase the number of elongated circular molecules observed in the AFM images.
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收藏
页码：824 / 828
页数：5
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