RETINAL GANGLION-CELLS EXPRESS A CGMP-GATED CATION CONDUCTANCE ACTIVATABLE BY NITRIC-OXIDE DONORS

被引：230
作者：
AHMAD, I
LEINDERSZUFALL, T
KOCSIS, JD
SHEPHERD, GM
ZUFALL, F
BARNSTABLE, CJ
机构：
[1] YALE UNIV, SCH MED, DEPT NEUROL, NEW HAVEN, CT 06510 USA
[2] YALE UNIV, SCH MED, NEUROBIOL SECT, NEW HAVEN, CT 06510 USA
来源：
NEURON | 1994年 / 12卷 / 01期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1016/0896-6273(94)90160-0
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
We have identified a putative cGMP-gated cation conductance in rat retinal ganglion cells. Both in situ hybridization and polymerase chain reaction amplification detected transcripts in ganglion cells that were highly homologous to the cGMP-gated cation channel expressed in rod photoreceptors. Whole-cell patch-clamp recordings detected a current stimulated by cGMP due to activation of nonselective cation channels. This current had a reversal potential near 0 mV, showed some outward rectification, and could be blocked by Cd2+. The current could also be activated by a phosphodiesterase inhibitor and the nitric oxide donors sodium nitroprusside and S-nitrosocysteine. We propose that nitric oxide released from an identified subpopulation of amacrine cells may activate this channel to modulate ganglion cell activity.
引用
收藏
页码：155 / 165
页数：11
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