A SIMPLE METHOD FOR SITE-DIRECTED MUTAGENESIS USING THE POLYMERASE CHAIN-REACTION

被引：546

作者：

HEMSLEY, A

ARNHEIM, N

TONEY, MD

CORTOPASSI, G

GALAS, DJ

机构：

[1] UNIV SO CALIF,DEPT MOLEC BIOL,LOS ANGELES,CA 90089

[2] UNIV CALIF BERKELEY,DEPT BIOCHEM,BERKELEY,CA 94720

来源：

NUCLEIC ACIDS RESEARCH | 1989年 / 17卷 / 16期

关键词：

D O I：

10.1093/nar/17.16.6545

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：6545 / 6551

页数：7

共 16 条

[1]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[2] NOVEL NON-TEMPLATED NUCLEOTIDE ADDITION-REACTIONS CATALYZED BY PROCARYOTIC AND EUKARYOTIC DNA-POLYMERASES [J].

CLARK, JM .

NUCLEIC ACIDS RESEARCH, 1988, 16 (20) :9677-9686

[3] A SIMPLE AND EFFICIENT PROCEDURE FOR SATURATION MUTAGENESIS USING MIXED OLIGODEOXYNUCLEOTIDES [J].

DERBYSHIRE, KM ;

SALVO, JJ ;

GRINDLEY, NDF .

GENE, 1986, 46 (2-3) :145-152

[4] A GENERAL-METHOD OF INVITRO PREPARATION AND SPECIFIC MUTAGENESIS OF DNA FRAGMENTS - STUDY OF PROTEIN AND DNA INTERACTIONS [J].

HIGUCHI, R ;

KRUMMEL, B ;

SAIKI, RK .

NUCLEIC ACIDS RESEARCH, 1988, 16 (15) :7351-7367

[5] AMPLIFICATION OF HUMAN MINISATELLITES BY THE POLYMERASE CHAIN-REACTION - TOWARDS DNA FINGERPRINTING OF SINGLE CELLS [J].

JEFFREYS, AJ ;

WILSON, V ;

NEUMANN, R ;

KEYTE, J .

NUCLEIC ACIDS RESEARCH, 1988, 16 (23) :10953-10971

[6] USE OF POLYMERASE CHAIN-REACTION CATALYZED BY TAQ-DNA POLYMERASE FOR SITE-SPECIFIC MUTAGENESIS [J].

KADOWAKI, H ;

KADOWAKI, T ;

WONDISFORD, FE ;

TAYLOR, SI .

GENE, 1989, 76 (01) :161-166

[7] THE GAPPED DUPLEX DNA APPROACH TO OLIGONUCLEOTIDE-DIRECTED MUTATION CONSTRUCTION [J].

KRAMER, W ;

DRUTSA, V ;

JANSEN, HW ;

KRAMER, B ;

PFLUGFELDER, M ;

FRITZ, HJ .

NUCLEIC ACIDS RESEARCH, 1984, 12 (24) :9441-9456

[8]

Maniatis T., 1982, MOL CLONING

[9]

MULLIS KB, 1987, METHOD ENZYMOL, V155, P335

[10]

OCHMAN H, 1988, GENETICS, V120, P621

← 1 2 →