NEW ELECTROPHORETIC METHOD FOR THE COMPLETE SEPARATION OF ALL KNOWN ANIMAL GLYCOSAMINOGLYCANS IN A MONODIMENSIONAL RUN

被引：175

作者：

CAPPELLETTI, R

DELROSSO, M

CHIARUGI, VP

机构：

[1] Institute of General Pathology, Florence University, School of Medicine, Firenze

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 99卷 / 02期

关键词：

D O I：

10.1016/S0003-2697(79)80012-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new rapid, sensitive, and reproducible method is presented which allows the separation of all known animal glycosaminoglycans (including two subclasses of heparan sulfate, chondroitin 4-sulfate, chondroitin 6-sulfate, dermatan sulfate, hyaluronic acid, heparin, and keratan sulfate) in a single monodimensional electrophoresis on cellulose acetate. A very narrow starting band is obtained by formation of a discontinuity in the electric field and all eight compounds are then selectively resolved by combining their migration properties in a barium acetate buffer and their differential sensitivity to precipitation by ethanol. © 1979 Academic Press, Inc.

引用

页码：311 / 315

页数：5

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