AUTOMATED-DETERMINATION OF FREE PHENYTOIN IN HUMAN PLASMA WITH ONLINE EQUILIBRIUM DIALYSIS AND COLUMN-SWITCHING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：17

作者：

ANDRESEN, AT ^{[1
]}

RASMUSSEN, KE ^{[1
]}

RUGSTAD, HE ^{[1
]}

机构：

[1] NATL HOSP NORWAY,RIKSHOSP,DEPT CLIN PHARMACOL,N-0027 OSLO,NORWAY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 621卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)80095-L

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Free phenytoin in human plasma was automatically determined by on-line equilibrium dialysis using the automated sequential trace enrichment of dialysate (ASTED) sample preparation system and HPLC. The dialysis cell was a modification of the cell supplied with the ASTED. Total phenytoin was analysed with the same analytical set-up and plasma protein binding was determined. Free phenytoin was determined in plasma from epileptic patients and the results were compared to those obtained by ultrafiltration. Automated determination of free and total phenytoin in plasma by the ASTED-HPLC combination was shown to be an accurate and reproducible method and the results in free phenytoin analyses were in agreement with those found with ultrafiltration. The sample throughput with the automated on-line combination of dialysis and column-switching HPLC was 75 samples in 24 h when the sample was dialysed at 37 degrees C.

引用

页码：189 / 198

页数：10

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