HISTAMINE OXIDASE, A CU2+-QUINOPROTEIN ENZYME OF ARTHROBACTER-GLOBIFORMIS

被引：16

作者：

SHIMIZU, E ^{[1
]}

ODAWARA, T ^{[1
]}

TANIZAWA, K ^{[1
]}

YORIFUJI, T ^{[1
]}

机构：

[1] OSAKA UNIV, INST SCI & IND RES, IBARAKI, OSAKA 567, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 11期

关键词：

D O I：

10.1271/bbb.58.2118

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Histamine oxidase (EC class 1.4.3) was found in cells of Arthrobacter globiformis IFO 12137 (ATCC 8010) grown on histamine, The enzyme purified to a specific activity of 9.4 units/mg had a purity of at least 80%. The enzyme oxidized histamine with concomitant formation of H2O2. Phenylethylamine, dopamine, aromatic monoamines, and aliphatic mono- and diamines were oxidized at lower rates. Cu2+-chelators inhibited the enzyme, indicating that the enzyme is Cu2+-dependent. Carbonyl-blocking reagents also inhibited the enzyme. The enzyme catalyzed the Nitro Blue Tetrazolium/glycinate reaction, which is characteristically catalyzed by quinones and quinoproteins. These results strongly suggest that the enzyme contains a quinonoid cofactor.

引用

页码：2118 / 2120

页数：3

共 12 条

[1] BROWN DE, 1991, J BIOL CHEM, V266, P4049
[2] EVIDENCE FOR COPPER AND 3,4,6-TRIHYDROXYPHENYLALANINE QUINONE COFACTORS IN AN AMINE OXIDASE FROM THE GRAM-NEGATIVE BACTERIUM ESCHERICHIA-COLI K-12
COOPER, RA
KNOWLES, PF
BROWN, DE
MCGUIRL, MA
DOOLEY, DM
[J]. BIOCHEMICAL JOURNAL, 1992, 288 : 337 - 340
[3] A NEW REDOX COFACTOR IN EUKARYOTIC ENZYMES - 6-HYDROXYDOPA AT THE ACTIVE-SITE OF BOVINE SERUM AMINE OXIDASE
JANES, SM
MU, D
WEMMER, D
SMITH, AJ
KAUR, S
MALTBY, D
BURLINGAME, AL
KLINMAN, JP
[J]. SCIENCE, 1990, 248 (4958) : 981 - 987
[4] LOWRY OH, 1951, J BIOL CHEM, V193, P265
[5] McIntire W. S., 1993, PRINCIPLES APPL QUIN, P97
[6] MCINTIRE WS, 1990, METHOD ENZYMOL, V188, P227
[7] PAZ MA, 1991, J BIOL CHEM, V266, P689
[8] PHENYLETHYLAMINE OXIDASE, A NOVEL ENZYME OF ARTHROBACTER-GLOBIFORMIS, MAY BE A QUINOPROTEIN
SHIMIZU, E
ICHISE, H
YORIFUJI, T
[J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1990, 54 (03): : 851 - 853
[9] VANIERSEL J, 1986, EUR J BIOCHEM, V161, P415
[10] VLIEGENTHART JFG, 1992, ENZYME NOMENCLATURE, P90

← 1 2 →