REACTIVATION OF METAL-REQUIRING APOENZYMES BY PHYTOCHELATIN METAL-COMPLEXES

被引：48
作者：
THUMANN, J
GRILL, E
WINNACKER, EL
ZENK, MH
机构：
[1] UNIV MUNICH,LEHRSTUHL PHARMAZEUT BIOL,KARLSTR 29,W-8000 MUNICH 2,GERMANY
[2] UNIV MUNICH,GENZENTRUM,W-8033 MARTINSRIED,GERMANY
来源：
FEBS LETTERS | 1991年 / 284卷 / 01期
关键词：
METALLO-ENZYME; PHYTOCHELATIN; METAL TRANSFER; REACTIVATION; HOMEOSTASIS;
D O I：
10.1016/0014-5793(91)80763-S
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The enzymatically inactive, metal-requiring apoforms of diamino oxidase and of carbonic anhydrase were reactivated by copper- and zinc-phytochelatin complexes, respectively. The level and the rate of reactivation effected by metal complexes consisting of poly(gamma-glutamylcysteinyl)glycine as well as by the respective free metal ion were compared. An efficient transfer of zinc and copper from phytochelatin-complexes to apoenzymes was observed in vitro.
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页码：66 / 69
页数：4
相关论文
共 16 条
[1]   METALLOTHIONEIN AND THE TRACE MINERALS [J].
BREMNER, I ;
BEATTIE, JH .
ANNUAL REVIEW OF NUTRITION, 1990, 10 :63-83
[2]   PURIFICATION AND CHARACTERIZATION OF DIAMINE OXIDASE FROM CLOVER LEAVES [J].
DELHAIZE, E ;
WEBB, J .
PHYTOCHEMISTRY, 1987, 26 (03) :641-643
[3]   PHYTOCHELATINS - THE PRINCIPAL HEAVY-METAL COMPLEXING PEPTIDES OF HIGHER-PLANTS [J].
GRILL, E ;
WINNACKER, EL ;
ZENK, MH .
SCIENCE, 1985, 230 (4726) :674-676
[4]   PHYTOCHELATINS, THE HEAVY-METAL-BINDING PEPTIDES OF PLANTS, ARE SYNTHESIZED FROM GLUTATHIONE BY A SPECIFIC GAMMA-GLUTAMYLCYSTEINE DIPEPTIDYL TRANSPEPTIDASE (PHYTOCHELATIN SYNTHASE) [J].
GRILL, E ;
LOFFLER, S ;
WINNACKER, EL ;
ZENK, MH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (18) :6838-6842
[5]  
GRILL E, 1988, PLANT CELL REP, V7, P375, DOI 10.1007/BF00269516
[6]   PHYTOCHELATINS, A CLASS OF HEAVY-METAL-BINDING PEPTIDES FROM PLANTS, ARE FUNCTIONALLY ANALOGOUS TO METALLOTHIONEINS [J].
GRILL, E ;
WINNACKER, EL ;
ZENK, MH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (02) :439-443
[7]  
GRILL E, 1991, IN PRESS METHODS ENZ
[8]   KINETICS OF BINDING OF ZINC(2) BY APOCARBONIC ANHYDRASE [J].
HENKENS, RW ;
STURTEVA.JM .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1968, 90 (10) :2669-&
[9]   RAPID AND CONVENIENT PREPARATION OF APOCARBONIC ANHYDRASE [J].
HUNT, JB ;
RHEE, MJ ;
STORM, CB .
ANALYTICAL BIOCHEMISTRY, 1977, 79 (1-2) :614-617
[10]   THE OXIDATION OF AMINES BY EXTRACTS OF PEA SEEDLINGS [J].
KENTEN, RH ;
MANN, PJG .
BIOCHEMICAL JOURNAL, 1952, 50 (03) :360-369
12