THE USE OF SITE-DIRECTED MUTAGENESIS AND TIME-RESOLVED FLUORESCENCE SPECTROSCOPY TO ASSIGN THE FLUORESCENCE CONTRIBUTIONS OF INDIVIDUAL TRYPTOPHAN RESIDUES IN BACILLUS-STEAROTHERMOPHILUS LACTATE-DEHYDROGENASE

被引：29
作者：
WALDMAN, ADB
CLARKE, AR
WIGLEY, DB
HART, KW
CHIA, WN
BARSTOW, D
ATKINSON, T
MUNRO, I
HOLBROOK, JJ
机构：
[1] PUBL HLTH LAB SERV,CTR APPL MICROBIOL & RES,MICROBIAL TECHNOL LAB,SALISBURY SP4 0JG,WILTS,ENGLAND
[2] SERC,DARESBURY LAB,WARRINGTON WA4 4AD,CHESHIRE,ENGLAND
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1987年 / 913卷 / 01期
关键词：
D O I：
10.1016/0167-4838(87)90233-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
引用
收藏
页码：66 / 71
页数：6
相关论文
共 21 条
[1]   VECTORS BEARING A HYBRID TRP-LAC PROMOTER USEFUL FOR REGULATED EXPRESSION OF CLONED GENES IN ESCHERICHIA-COLI [J].
AMANN, E ;
BROSIUS, J ;
PTASHNE, M .
GENE, 1983, 25 (2-3) :167-178
[2]  
Bevington P.R., 1969, DATA REDUCTION ANAL, V3rd edn
[3]   SITE-DIRECTED MUTAGENESIS REVEALS ROLE OF MOBILE ARGININE RESIDUE IN LACTATE-DEHYDROGENASE CATALYSIS [J].
CLARKE, AR ;
WIGLEY, DB ;
CHIA, WN ;
BARSTOW, D ;
ATKINSON, T ;
HOLBROOK, JJ .
NATURE, 1986, 324 (6098) :699-702
[4]   CHANGES IN THE STATE OF SUBUNIT ASSOCIATION OF LACTATE-DEHYDROGENASE FROM BACILLUS-STEAROTHERMOPHILUS [J].
CLARKE, AR ;
WALDMAN, ADB ;
MUNRO, I ;
HOLBROOK, JJ .
BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 828 (03) :375-379
[5]   THE ASSEMBLY MECHANISM OF THE LACTATE-DEHYDROGENASE TETRAMER FROM BACILLUS-STEAROTHERMOPHILUS - THE EQUILIBRIUM RELATIONSHIPS BETWEEN QUATERNARY STRUCTURE AND THE BINDING OF FRUCTOSE-1,6-BISPHOSPHATE, NADH AND OXAMATE [J].
CLARKE, AR ;
ATKINSON, T ;
CAMPBELL, JW ;
HOLBROOK, JJ .
BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 829 (03) :387-396
[6]  
Gibson T.J., 1984, THESIS U CAMBRIDGE
[7]   PROTEIN FLUORESCENCE OF LACTATE-DEHYDROGENASE [J].
HOLBROOK, JJ .
BIOCHEMICAL JOURNAL, 1972, 128 (04) :921-&
[8]  
Holbrook JJ, 1975, ENZYMES A, VXI, P191
[9]  
JECSAI G, 1962, ACTA PHYSL ACAD SCI, V20, P339
[10]  
LAKOWICZ JR, 1980, J BIOL CHEM, V255, P831
123