STATISTICAL EVALUATION OF THE VALIDITY OF A METHOD FOR CHARACTERIZING STATIONARY PHASES FOR REVERSED-PHASE LIQUID-CHROMATOGRAPHY BASED ON RETENTION OF HOMOLOGOUS SERIES

被引：9

作者：

BREUER, MRP ^{[1
]}

CLAESSENS, HA ^{[1
]}

CRAMERS, CA ^{[1
]}

机构：

[1] EINDHOVEN UNIV TECHNOL,INSTRUMENTAL ANAL LAB,5600 MB EINDHOVEN,NETHERLANDS

来源：

CHROMATOGRAPHIA | 1994年 / 38卷 / 3-4期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; CHARACTERIZATION OF REVERSED PHASES; COLUMN HYDROPHOBICITY; COLUMN HYDROPHILICITY;

D O I：

10.1007/BF02290326

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The validity of a method for characterizing stationary phases for reversed-phase, liquid chromatography, based on the use of homologous series, has been evaluated. The method is based on a retention model which describes the dependence of the logarithm of the capacity factor on mobile phase composition and the carbon number of specific homologous series. A first-order as well as a second-order version of this model was investigated. The second-order model proved to be a significant improvement on the first-order model, even for smaller mobile-phase ranges. Nevertheless both models showed a significant lack of fit, reflecting the incompleteness of these models. Therefore, it is very questionable whether this method is suitable to describe HPLC-column characteristics like hydrophobicity and hydrophylicity.

引用

页码：137 / 146

页数：10

共 12 条

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