STATISTICAL EVALUATION OF THE VALIDITY OF A METHOD FOR CHARACTERIZING STATIONARY PHASES FOR REVERSED-PHASE LIQUID-CHROMATOGRAPHY BASED ON RETENTION OF HOMOLOGOUS SERIES

被引：9

作者：

BREUER, MRP ^{[1
]}

CLAESSENS, HA ^{[1
]}

CRAMERS, CA ^{[1
]}

机构：

[1] EINDHOVEN UNIV TECHNOL,INSTRUMENTAL ANAL LAB,5600 MB EINDHOVEN,NETHERLANDS

来源：

CHROMATOGRAPHIA | 1994年 / 38卷 / 3-4期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; CHARACTERIZATION OF REVERSED PHASES; COLUMN HYDROPHOBICITY; COLUMN HYDROPHILICITY;

D O I：

10.1007/BF02290326

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The validity of a method for characterizing stationary phases for reversed-phase, liquid chromatography, based on the use of homologous series, has been evaluated. The method is based on a retention model which describes the dependence of the logarithm of the capacity factor on mobile phase composition and the carbon number of specific homologous series. A first-order as well as a second-order version of this model was investigated. The second-order model proved to be a significant improvement on the first-order model, even for smaller mobile-phase ranges. Nevertheless both models showed a significant lack of fit, reflecting the incompleteness of these models. Therefore, it is very questionable whether this method is suitable to describe HPLC-column characteristics like hydrophobicity and hydrophylicity.

引用

页码：137 / 146

页数：10

共 12 条

[1] CHARACTERIZATION OF CHEMICALLY MODIFIED SILICA-GELS BY SI-29 AND C-13 CROSS-POLARIZATION AND MAGIC ANGLE SPINNING NUCLEAR MAGNETIC-RESONANCE [J].

BAYER, E ;

ALBERT, K ;

REINERS, J ;

NIEDER, M ;

MULLER, D .

JOURNAL OF CHROMATOGRAPHY, 1983, 264 (02) :197-213

[2] CHROMATOGRAPHIC AND SOLID-STATE NUCLEAR MAGNETIC-RESONANCE STUDY OF THE CHANGES IN REVERSED-PHASE PACKINGS FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AT DIFFERENT ELUENT COMPOSITIONS [J].

CLAESSENS, HA ;

DEHAAN, JW ;

VANDEVEN, LJM ;

DEBRUYN, PC ;

CRAMERS, CA .

JOURNAL OF CHROMATOGRAPHY, 1988, 436 (03) :345-365

[3]

DEMING SV, 1984, NATO AST SERIES C, V1138

[4]

HETEM MJJ, 1990, THESIS EINDHOVEN U T

[5] A METHOD FOR CHARACTERIZATION AND OPTIMIZATION OF REVERSED-PHASE LIQUID-CHROMATOGRAPHIC SEPARATIONS BASED ON THE RETENTION BEHAVIOR IN HOMOLOGOUS SERIES [J].

JANDERA, P .

CHROMATOGRAPHIA, 1984, 19 :101-112

[6] REVERSED-PHASE LIQUID-CHROMATOGRAPHY OF HOMOLOGOUS SERIES - A GENERAL-METHOD FOR PREDICTION OF RETENTION [J].

JANDERA, P .

JOURNAL OF CHROMATOGRAPHY, 1984, 314 (NOV) :13-36

[7] METHOD FOR CHARACTERIZATION OF SELECTIVITY IN REVERSED-PHASE LIQUID-CHROMATOGRAPHY .1. DERIVATION OF THE METHOD AND VERIFICATION OF THE ASSUMPTIONS [J].

JANDERA, P .

JOURNAL OF CHROMATOGRAPHY, 1986, 352 :91-110

[8]

LEACH DC, 1988, LC GC, V6, P494

[9]

Massart D. L., 1988, CHEMOMETRICS TXB

[10] SOLVATOCHROMIC HYDROGEN-BOND DONOR ACIDITY OF AQUEOUS BINARY SOLVENT MIXTURES FOR REVERSED-PHASE LIQUID-CHROMATOGRAPHY [J].

PARK, JH ;

JANG, MD ;

KIM, DS ;

CARR, PW .

JOURNAL OF CHROMATOGRAPHY, 1990, 513 :107-116

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