ULTRASTRUCTURAL IN-SITU HYBRIDIZATION AND AUTORADIOGRAPHIC DETECTION OF FOREIGN DNA IN ZEBRAFISH SPERMATOZOA

被引：0
|
作者：
PATIL, JG
KHOO, HW
机构：
来源：
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1995年 / 35卷 / 05期
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中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Conditions have been established which permit specific hybridization of foreign DNA to complimentary DNA in ultra thin sections of zebrafish sperm prepared for electron microscopy (EM)(1) after fixation in 2% paraformaldehyde, 2.5% glutaraldehyde and embedded in spurs resin. Zebrafish sperm incubated with foreign DNA were subjected to ultrastructural in situ hybridization and autoradiographic detection to determine if the sperm could retain and internalize the foreign DNA sequences. Specific and intense signals were detected from the sperm head indicating that the zebrafish sperm are capable of internalizing foreign DNA.
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页码：965 / 969
页数：5
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